The Journal of Neuroscience, January 5, 2005, ():
Impaired Channel Targeting and Retinal Degeneration in Mice Lacking the Cyclic Nucleotide-Gated Channel Subunit CNGB1
J. Neurosci. Hüttl et al.
25: 130
Supplemental data
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Fig. S1. Normal retinal morphology in CNGB1+/- mice. Normal expression of CNGB1 (A) and CNGA1 (B) in rod outer segments of PW8 heterozygous mice. (C) Presence of rods and cones in PM9 CNGB1+/- mice. Rods and cones were visualized with anti-rhodopsin (red) antibody and PNA (green), respectively. Cell nuclei were stained with Hoechst dye (blue). (D). Absence of gliosis and normal expression pattern of GFAP in PM9 CNGB1+/- mice. (E, F) Toluidine blue-stained semithin section of a PM4 CNGB1+/- mouse retina compared to a retinal section of a age-matched CNGB1+/+ mouse. (G) Western blot analysis of CNGB1 expression using an antibody directed against the N-terminus of CNGB1a (GARP antibody, PPc6N). The antibody detects a 240 kD protein in membrane fractions of HEK293 cells expressing recombinant CNGB1a, in CNGB1+/+ and in CNGB1+/- mice. In CNGB1-/- mice no protein is detected. In the last lane membrane fractions from HEK293 cells expressing a truncated version of the CNGB1a protein encoded by exon 1 to 25 were probed by the antibody. No truncated protein is found in CNGB1+/- and CNGB1-/- mice. The bar at the right side of the panel indicates the position of a 220 kD size marker. Scale bars indicate 20µm in (A-C), 100µm in (D) and 50µm in (E-F).
