The Journal of Neuroscience, January 5, 2005, ():
A Functional Interaction of Sodium and Calcium in the Regulation of NMDA Receptor Activity by Remote NMDA Receptors
J. Neurosci. Xin et al.
25: 139
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Fig.1. Changes in [Na+]i and [Ca2+]i induced by bath-applied L-aspartate in neurons bathed with extracellular solution containing 20mM Na+ and the Na+ ionophor, monensin (10mM).
A, an example of [Na+]i recorded in the soma region of neurons bathed initially with a standard extracellular solution containing 200mM Na+. Application of the extracellular solution containing monensin and 0 Na+ to the neurons significantly decreased [Na+]i. Subsequent increase of [Na+] to 20mM in the bath solution containing monensin significantly increased [Na+]i. L-aspartate application to neurons bathed with extracellular solution containing 20mM Na+ and monensin produced a significant reduction in [Na+]i. B, an example of [Ca2+]i recorded in the soma region of neurons bathed in the extracellular solution containing 20mM Na+ and monensin before and during L-aspartate application. Summarized data outlining the effects of L-aspartate application are shown in Fig.5D.
- supplemental material
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Supplementary Fig.2. Changes in [Na+]i and [Ca2+]i induced by increasing K+ concentration to 30mM in extracellular bath solution.
A and C respectively show examples of [Na+]i and [Ca2+]i recorded from neurons bathed with a standard extracellular solution before and after increasing [K+]e to 30mM. B and D show summarized data (meanąSEM) outlining increases in [Na+]i and [Ca2+]i induced by increasing [K+]e.
