The Journal of Neuroscience, March 23, 2005, ():
An Essential Drosophila Glutamate Receptor Subunit That Functions in Both Central Neuropil and Neuromuscular Junction
J. Neurosci. Featherstone et al.
25: 3199
Supplemental data
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Figure S1: Expression of glutamate receptor subunits in mutant NMJs. Confocal images showing intersegmental nerve branch b (ISNb), visualized using anti-HRP antibody (neuronal membrane epitope, magenta), innervating several ventral longitudinal muscles. A: At control NMJs (top row), glutamate receptors visualized using an anti-GluRIIC antibody (green) form distinct clusters opposite presynaptic terminals. In homozygous brec mutants (second row), there is no detectable GluRIIC immunoreactivity. B: At control NMJs (top row), glutamate receptors visualized using an anti-GluRIIB antibody (green) form distinct clusters opposite presynaptic terminals. In homozygous brec mutants (second row), there is no detectable GluRIIB immunoreactivity. C: At control NMJs (top row), glutamate receptors visualized using an anti-GluRIID antibody (green) form distinct clusters opposite presynaptic terminals. In homozygous Df(2L)SP22 mutants (second row), which is a deletion that removes both GluRIIA and GluRIIB, there is no detectable GluRIID immunoreactivity. D: At control NMJs (top row), glutamate receptors visualized using an anti-GluRIIA antibody (green) form distinct clusters opposite presynaptic terminals. In transheterozygous brec mutants (second row), there is no detectable GluRIIA immunoreactivity.
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