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Figure 6. Quantification of SNB-1::GFP and SYD-2::GFP puncta during development. Using Punc-25-DsRed to label GABAergic neurons, SYD-2::GFP (hpIs3) puncta were counted in the dorsal cord region between the commissures of motoneurons VD10 and VD12. A-C, Examples of DsRed-labeled commissures meeting the dorsal cord (arrows). Scale bar, 10 µm. D, Schematic representation of this region (courtesy of E. Jorgensen). E, The results of SNB-1::GFP (juIs1) and SYD-2::GFP (hpIs3) puncta quantification within this region (as shown in A-D) are illustrated by dark filled bars. Puncta were also quantified by counting the number of puncta within a 50 µm distance at different stages during C. elegans development. These results are shown as shaded bars. Numbers for juIs1 puncta are shown in yellow, and numbers for hpIs3 puncta are shown in blue.
