The Journal of Neuroscience, May 4, 2005, ():
Schwann Cell-Specific Ablation of Laminin 
1 Causes Apoptosis and Prevents Proliferation
J. Neurosci. Yu et al.
25: 4463
Supplemental data
Files in this Data Supplement:
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Figure S1. Schwann cells with laminin ?1 surrounding them in mutant sciatic nerves expresses Cre.
Mice heterozygous for the fLAM?1 allele and hemizygous for the P0Cre transgene were crossed with mice homozygous for the fLAM?1 allele and hemizygous for a Cre reporter transgene, Z/EG (lacZ/EGFP) to obtain P0/Cre:fLAM ?1//Z/EG mice (f/f, P0-Cre, Z/EG) mice, P0/Cre:fLAM ?1/+//Z/EG mice (f/+, P0-Cre, Z/EG, positive control) and fLAM ?1//Z/EG mice (f/f, Z/EG, negative control). This Z/EG Cre reporter transgene activates expression of EGFP upon Cre-mediated gene recombination. A, Transverse sections of sciatic nerves from P0/Cre:fLAM ?1//Z/EG mice at P28 were stained for laminin ?1 (red) and counterstained with DAPI (blue). EGFP expression was directly visualized under fluorescent microscopy. Some Schwann cells in the sciatic nerve of the mutant mice that also carry the reporter gene have laminin ?1 surrounding them and expressed EGFP by Cre-mediated recombination (arrows). This result indicates that these Schwann cells expressed Cre, but may have obtained laminin ?1 from other cellular sources. B, Transverse sciatic nerve sections from P0/Cre:fLAM ?1/+//Z/EG mice and fLAM ?1//Z/EG mice at P28 served as positive (f/+, P0-Cre, Z/EG) and negative controls (f/f, Z/EG) for EGFP expression. Bar: 2 ?m.
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Figure S2. Increased Schwann cells apoptosis in later developmental and adult stages in CaMKII/Cre:fLAM?1 mutant mice.
A, Representative longitudinal sciatic nerve sections of control and mutant mice at P5 and transverse sections of control and mutant mice at P10 and adult were stained with TUNEL (green) and counterstained with laminin ?1 (red) and the images were merged. Apoptotic cells can be detected in mutant sciatic nerve at all stages (arrows) and in control nerves at P5, but barely detectable in control nerves at P10 and adult. B, Plot of the percentage of TUNEL-positive nuclei (mean?SEM) at various ages. The percentages of TUNEL-positive nuclei were higher in mutant (open bar) than in control nerves (close bar) at P10 and adult (n=6 per genotype per age; ** P<0.001), but there was no significant difference at P5.
