The Journal of Neuroscience, May 11, 2005, ():
A Noncanonical Release of GABA and Glutamate Modulates Neuronal Migration
J. Neurosci. Manent et al.
25: 4755
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Figure S1: Birthdating analysis of CA1 pyramidal cells and receptors expression in the migration area.
(A) Histograms showing the number of BrdU-positive cells per mm3, detected into the stratum pyramidale of the CA1 region of P7-8 and P14-15 animals, after single BrdU injection during embryonic development from E12 to E19. Each data point represents mean ± SEM (3 animals and 9 sections per age).
(B) Immunostaining for BrdU on hippocampal sections, revealing the inside-out construction of the stratum pyramidale (s. pyr.). Top left panel: animal injected at E12 and perfused at P7-8. The BrdU-positive cells are located into the deepest part of the stratum pyramidale, close to the stratum oriens (s. or.). Top right panel: animal injected at E12 and perfused at P14-15. The BrdU-positive cells are located into the deepest part of the stratum pyramidale, close to the stratum oriens. Bottom left panel: animal injected at E18 and perfused at P7-8. The BrdU-positive cells are located into the superficial part of the stratum pyramidale, close to the stratum radiatum (s. rad.). Bottom right panel: animal injected at E18 and perfused at P14-15. The BrdU-positive cells are located into the superficial part of the stratum pyramidale, close to the stratum radiatum. Scale bar: 10 µm.
(C) Immunostaining of E18 hippocampal sections, revealing the presence of the GluR2/3 subunits of the AMPA receptor (left panel), the NR1 subunit of the NMDA receptor (middle panel) and the GABAA receptors (right panel), throughout the migration area – the neuroepithelium (ne) and the stratum oriens. Scale bar: 10 µm.
