The Journal of Neuroscience, May 18, 2005, ():
Estradiol Activates Group I and II Metabotropic Glutamate Receptor Signaling, Leading to Opposing Influences on cAMP Response Element-Binding Protein
J. Neurosci. Boulware et al.
25: 5066
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1: Inhibition of calcineurin with FK506 (200 ng/ml) and cyclosporin A (1 µg/ml) does not alter estradiol-induced CREB phosphorylation (F=17.36; **p<0.01 vs. no steroid ±FK506/CsA).
- supplemental material
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Supplemental Figure 2: LY367385 does not dampen all Gq signaling, as demonstrated by the increase in CREB phosphorylation following application of the muscarinic receptor agonist pilocarpine (1 µM) (F=120.10; **p<0.01 in comparison to baseline with/without LY367385).
- supplemental material
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Supplemental Figure 3: The mGluR2/3-induced reduction in L-type calcium channel mediated CREB phosphorylation is mediated through Gi/o and PKA signaling. (A) Pretreatment with Pertussis toxin (500 ng/ml) blocked the APDC-induced attenuation in CREB phosphorylation (F=112.29; **p<0.01) (B) Inhibition of PKA with Rp-cAMPs (50 µM) occluded the effect of APDC on L-type calcium channel-mediated CREB phosphorylation (F=64.97; **p<0.01).
- supplemental material
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Supplemental Figure 4: Expression of ER? and ER? in hippocampal cultures derived from both male and female rats. (A) Top: Paired, low magnification photomicrographs of ER? (red) and ?-tubulin (green) in cultured hippocampal neurons from female animals. Middle: Higher magnification of ER? and ?-tubulin in neurons derived from both female and male animals. Bottom: Summary of ER? staining. (B) Similar to A, but ER? expression.
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