The Journal of Neuroscience, May 25, 2005, ():
Emx2 and Pax6 Function in Cooperation with Otx2 and Otx1 to Develop Caudal Forebrain Primordium That Includes Future Archipallium
J. Neurosci. Kimura et al.
25: 5097
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplemental Fig. 1
Analysis of E12.5 Emx2-/-Otx1-/- double mutant phenotype with diencephalic markers indicated. Caudal forebrain structures are greatly reduced. All are sagittal views. Scale bar is 500 µm.
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Supplemental Fig. 2
Generation of Emx2+/Cre mice. (A) schematic diagram of knock-in strategy. Thick and thin lines indicate Emx2 genomic and plasmid-derived sequences, respectively; black boxes, exons; black triangles, loxP sequences. The predicted sizes of wild and knock-in alleles and the probes used in Southern blot analysis shown in (B) are indicated. E, EcoRI; N, NotI; S, SalI. (B) examples of Southern blot analysis by EcoRI digestion; upper is an example of the analysis in homologous recombinant ES cells with probe 1, and lower that in knock-in mutant mice with probe 2.
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Supplemental Fig. 3
Comparison of diencephalic marker phenotypes among Pax6-/- single, Emx2-/-Pax6-/- double and Emx1-/-Emx2-/-Pax6-/- triple mutants at E12.5. Markers and genotypes of embryos examined are indicated at left and top, respectively. In Pax6 single mutant, Pax6, Lim1 and Wnt8b-positive eminentia thalami, Pax6, Dlx1 and Lim1-positive prethalamus, Gbx2 and Tcf4-positive thalamus, Ebf1 and Tcf4-positive anterior pretectum are reduced, but present; Pax6 and Lim1-positive posterior pretectum exists scarcely (Stoykova et al., 1996; Warren and Price, 1997). These structures are completely lost in the Emx2-/-Pax6-/- double mutant. Emx1-/-Emx2-/-Pax6-/- triple mutant phenotype is the same as the double mutant one. Scale bar is 500 µm in (Aa, Ba, Ca, Da, Ha). See Fig. 6 legend for others.
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Supplemental Fig. 4
Comparison of telencephalic marker phenotypes among Pax6-/- single, Emx2-/-Pax6-/- double and Emx1-/-Emx2-/-Pax6-/- triple mutants at E12.5. Markers and genotypes of embryos examined are indicated at left and top, respectively. TTR-positive choroid plexus, Wnt3a-positive cortical hem and Ephb1-positive prospective hippocampus are present in the single mutants, though reduced; these are lost in the double mutant. Wnt8b-positive medial pallium is residual in the double mutant; it is deformed but present in the single mutant. Dlx1 expression is extended much more into the pallial region in the double mutant than in single mutant. Pax6, Emx1 and Ngn2-positive pallium are more meager in the double mutant than in the single mutant. Medially the double mutant pallium expresses roof genes, Lhx5 and Msx1. Emx1-/-Emx2-/-Pax6-/- triple mutant phenotype is the same as the double mutant one. Scale bars 240 µm in (Aa, Ea, Ja). See Fig. 7 legend for others.
