The Journal of Neuroscience, May 25, 2005, ():
Rhodopsin Formation in Drosophila Is Dependent on the PINTA Retinoid-Binding Protein
J. Neurosci. Wang and Montell
25: 5187
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Binding of retinoids to PINTA. Fluorometric titration of 0.1 ƒÝM solutions of His-tagged PINTA with various retinoids. A, all-trans retinoic acid. B, all-trans retinal. C, 11-cis retinal. D, 9-cis retinoic acid.
- supplemental material
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Supplemental Figure 2. Neither CRALBP nor CRBP-I restore normal function in pinta flies. A-D, PDAs performed as described in Fig. 1A. A, wild-type (w1118). B, pinta. C, Human CRALBP expressed in pinta flies under the control of the hsp70 promoter: P[hs-cralbp];pinta. D, Human C-terminal MYC-tagged CRBP-I expressed in pinta flies: P[hs-crbp-myc];pinta. E-F, Western blots showing restoration of low levels of Rh1. E, P[hs-cralbp];pinta and F, P[hs-crbp-myc];pinta. CRALBP and CRBP-I-MYC expression was determined by probing the Western blots with anti-CRALBP and anti-MYC antibodies respectively. The blots were also probed with anti-Tubulin antibodies to compare the levels of loading between lanes.
