The Journal of Neuroscience, June 8, 2005, ():
Chronic Nicotine Exposure Upregulates Nicotinic Receptors by a Novel Mechanism
J. Neurosci. Vallejo et al.
25: 5563
Supplemental Material
Files in this Data Supplement:
- Supplementary Fig. 1
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Figure S1. Montage of cell surface immunostaining of ?4?2HA expressing cells. The group of cells in the figure correspond tothe cells in Figure 1B. Intact cells were immunostained with anti-HA mAb HA.11. 1 ?m thick slices visualized by confocal microscopy are shown along with their respective DIC images (left). The bottom of the cell is located in the upper left corner and the top of the cell is at the bottom right. Scale bar is 20 ?m.
- Supplementary Fig. 2
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Figure S2. Montage of MTSEA-biotinylation and immunostaining of cell surface ?4?2HA receptors. A. The cell in the center of the figure corresponds to the cell in Figure 2A (left). B. A group of seven cells not shown in Figure 2. Biotinylated, intact cells (A and B) were stained with Alexafluor streptavidin (upper panel) and anti-HA mAb HA.11 (lower panel). Corresponding DIC images are on the left. Slices are 1 ?M thick. The bottom of the cell is located in the upper left corner and the top of the cell is at the bottom right. Scale bars are 5 ?m (A) and 10 ?m (B).
- Supplementary Fig. 3
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Figure S3. Montage of intracellular MTSEA-biotinylation. . A. The two cells in the figure correspond to the cells in Figure 2A (right). B. A group of five cells not shown in Figure 2. Cells that were permeabilized prior to biotinylation (A and B) were stained with Alexafluor streptavidin to visualize the intracellular distribution of MTSEA. Corresponding DIC images are on the left. 1 ?m slices are shown with the bottom of the cell located at the upper left and the top of the cell on the lower right. Scale bars are 5?m (A) and 10 ?m (B).
