The Journal of Neuroscience, June 15, 2005, ():
Nucleokinesis in Tangentially Migrating Neurons Comprises Two Alternating Phases: Forward Migration of the Golgi/Centrosome Associated with Centrosome Splitting and Myosin Contraction at the Rear
J. Neurosci. Bellion et al.
25: 5691
Supplemental data
Files in this Data Supplement:
- supplemental material -
Table1 : Mean speed of migration of MGE cells imaged on E13.5 isochronic dissociated cortical cells, and in E13.5 or E14.5 isochronic cortical slices. E13.5 MGE cells migrate significantly faster in slices than on dissociated cortical cells. In slices, the speed of migration of MGE cells increases from E13.5 to E14.5.
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Movie 2A : .
GFP-expressing MGE cells migrate on a layer of non fluorescent dissociated cortical cells. Cells are imaged each 3 minutes with a X20 objective during 100 minutes.
Nuclear translocations are preceded by the forward migration of cytoplasmic materiel located initially around the nucleus.
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Movie 3A
A GFP-expressing MGE cell grafted in the basal telencephalon of a forebrain slice migrates in the lower intermediate zone of the cortex. The slice was imaged each 3 minutes with a X20 objective during 2 hours and 35 minutes. The migration is saltatory. Each nuclear translocation is preceded by the forward migration of cytoplasmic material that forms a large swelling ahead the nucleus. The cell produces neuritic forks at the leading edge by splitting a leading growth cone.
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Movie 5B1
GFP-expressing MGE cells migrate on non fluorescent cortical neurons. Cells were imaged each 3 minutes with a X40 objective during 5 hours and 33 minutes. Blebbistatin 70 µM was applied at 20h35. Prior blebbistatin application, the nucleus shows two long distance translocations. Following blebbistatin application, nuclear translocations are lo longer observed, one neurite retracts and active lamellipodia form around the nucleus and at the fork.
