The Journal of Neuroscience, June 15, 2005, ():
Bmi1 Loss Produces an Increase in Astroglial Cells and a Decrease in Neural Stem Cell Population and Proliferation
J. Neurosci. Zencak et al.
25: 5774
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplement Figure 1 Bmi1-knockout mice show a normal telencephalic development at birth. The cross-sectional area of various regions of the telencephalon was measured at birth at four different levels (see methods). The histogram represents the mean area (in ?m2) of the 4 measured sections for the cortex (Cx), the striatum (Str), the SVZ, and the dorso-lateral corner of the SVZ (SVZc) (see methods for precisedefinition of the areas). No significant difference was observed between genotypes.
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Supplement Figure 2 Neurospheres express Bmi1 and LeX. Neurospheres were plated onto fibronectin in the presence of EGF and processed for immunocytochemistry 24 to 72 hours after sphere transfer. In such condition almost all cells derived from a neurophere express Bmi1 (a and b). (b), Higher magnification of the white area delimited in (a) showing that rare cells failed to express Bmi1 (arrows). (c) LeX is also expressed in numerous cells, but its expression is restricted to the core of the neurosphere. (d, e, f), DAPI staining of (a, b, c).Magnification: (a, c, d, f): 200x, (b, e): 800x.
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Supplement Figure 3 Apoptosis in the newborn brain. Apoptotic cells were identified by labeling fragmented DNA with the TUNEL method and analysed in the same regions as for the BrdU assay: the cortex (CX), the corpus callosum (CC), the subventricular zone (SVZ) and the ventricular zone (VZ) (a, b) Representative TUNEL labeling with the DAB chromogen (brown) in the dorso-medial corner of the SVZ (a) and in the cortex (b), where very rare apoptotic cells were detected. (c), Quantification of the TUNEL+ cells in the mentioned regions. No significant differences were observed between wild-type (WT), Bmi1+/- (HET) and Bmi1-/- (KO) mice details (n=3 for WT and Bmi1+/-, and n=4 animals for KO, p>0.05). (a, b): magnification 400x.
