The Journal of Neuroscience, June 22, 2005, ():
Fasciclin II Signals New Synapse Formation through Amyloid Precursor Protein and the Scaffolding Protein dX11/Mint
J. Neurosci. Ashley et al.
25: 5943
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1 Ultrastructure of buds in wild type, [FasII]-pre-post, and [APPL]-pre. (A-D) Cross sections through a parent type I bouton (P) and its neighboring buds (arrows) in (A-B) wild type, (C) [APPL]-pre, and (D) [FasII]-pre-post. Arrowheads point to synapses. SSR = subsynaptic reticulum. Scale Bar = 0.8 µm.
- supplemental material
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Supplementary Figure 2 Symmetrical changes in FasII levels enhance the extent of NMJ branching and bouton number in an APPL-dependent manner, and quantification of APPL levels in dX11 mutants. (A) Number of branches at NMJs of mutants with altered FasII and APPL levels. Number of samples quantified are wild type = 11; Appld = 11 , fasIIe76/+ = 11; Appld, fasIIe76/Appld, + = 12; [FasII]-pre-post (using Gal4 drivers: C164 and C57) = 11; [APPL]-pre (using Gal4 driver C164)= 11; [FasII, APPL]-pre-post (using Gal4 drivers C380 and BG487)= 10; Appld, [FasII]-pre-post (using Gal4 drivers: C164 and C57) = 8. (B) Number of boutons at muscles 6 and 7 in FasII and APPL variants. Number of samples quantified are wild type = 95, [APPL]-pre = 14, [FASII]-pre = 43, [FASII,APPL]-pre = 25, [FASII,APPLDC] = 10. (C) Percentage of APPL intensity levels inside the boutons of dX11 variants (see Methods). Number of samples quantified are 10 for each genotype. *** p<0.0001, * p<0.05.
