Figure 1.
β2-AR agonist induced translocation of PKCϵ in cultured DRG neurons. A, Confocal images of representative untreated (left) versus isoproterenol-treated (1 μm, 30 s; right) cultured DRG neurons. Cultures were fixed after treatment, incubated with the affinity-purified PKCϵ-specific antiserum SN134 (1:1000), and detected with FITC-coupled donkey anti-rabbit IgG serum (1:200). Insets, Enlarged area, indicated by the white frame. After isoproterenol treatment, a portion of PKCϵ can be seen to translocate to the plasma membrane. Scale bar, 20 μm. B, Percentage of neurons demonstrating, after 30 s, PKCϵ translocation to the plasma membrane in response to indicated concentrations of isoproterenol. C, Percentage of neurons demonstrating PKCϵ translocation with isoproterenol (1 μm) stimulation, for indicated time points. Filled bars represent cultures treated with 1 μm isoproterenol. Cultures represented by dotted bars were pretreated for 15 min with the β2-AR-specific inhibitor ICI 118,551 (50 μm) before stimulation with 1 μm isoproterenol. **p < 0.01. Error bars represent SEM.