The Journal of Neuroscience, June 29, 2005, ():
Sortilin Controls Intracellular Sorting of Brain-Derived Neurotrophic Factor to the Regulated Secretory Pathway
J. Neurosci. Chen et al.
25: 6156
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Co-immunoprecipitation of cytosolic sortilin (cytSort) with BDNF. HEK 293 cells were co-transfected with cDNA’s encoding a mutant cytoplasmic region sortilin fused with GFP, as described in Materials and Methods, along with carboxy terminal tagged BDNF-HA. Cell lysates were immunoprecipitated with HA antibodies and immunoblotted with GFP antibodies (top panel). Immunoprecipitation of HA-BDNF was confirmed by immunoblotting with HA antibodies (middle panel). Crude lysates were immunoblotted with GFP antibodies to confirm equivalent expression levels (bottom panel).
- supplemental material
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Supplemental Figure 2. pH dependence of BDNF-sortilin interaction. HEK 293 cells were co-transfected with cDNA’s encoding sortilin and carboxy terminal HA-BDNFVal. Cell lysates were adjusted to the indicated pH’s, and immunoprecipitated with HA antibodies and immunoblotted with sortilin antibodies (top panel). Immunoprecipitation of BDNF-HA was confirmed by immunoblotting with HA antibodies (middle panel). Crude lysates were immunoblotted with sortilin antibodies to confirm equivalent expression levels (bottom panel).
