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The Journal of Neuroscience, July 20, 2005, 25(29):6907-6910; doi:10.1523/JNEUROSCI.1905-05.2005
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BRIEF COMMUNICATION
Lack of NMDA Receptor Subtype Selectivity for Hippocampal Long-Term Potentiation
Sven Berberich,1 *
Pradeep Punnakkal,1 *
Vidar Jensen,2
Verena Pawlak,1
Peter H. Seeburg,1
Øivind Hvalby,2 and
Georg Köhr1
1Department of Molecular Neurobiology, Max-Planck-Institute for Medical Research, D-69120 Heidelberg, Germany, and 2Molecular Neurobiology Research Group, Institute of Basic Medical Sciences, University of Oslo, N-0317 Oslo, Norway
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Abstract
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NMDA receptor (NMDAR) 2A (NR2A)- and NR2B-type NMDARs coexist in synapses of CA1 pyramidal cells. Recent studies using pharmacological blockade of NMDAR subtypes proposed that the NR2A type is responsible for inducing long-term potentiation (LTP), whereas the NR2B type induces long-term depression (LTD). This contrasts with the finding in genetically modified mice that NR2B-type NMDARs induce LTP when NR2A signaling is absent or impaired, although compensatory mechanisms might have contributed to this result. We therefore assessed the contribution of the two NMDAR subtypes to LTP in mouse hippocampal slices by different induction protocols and in the presence of NMDAR antagonists, including the NR2A-type blocker NVP-AAM077, for which an optimal concentration for subtype selectivity was determined on recombinant and native NMDARs. Partial blockade of NMDA EPSCs by 40%, either by preferentially antagonizing NR2A- or NR2B-type NMDARs or by the nonselective antagonist D-AP-5, did not impair LTP, demonstrating that hippocampal LTP induction can be generated by either NMDAR subtype.
Key words: NVP-AAM077; PEAQX; CP-101,606; recombinant; gene-targeted mouse; partial blockade; pairing
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Introduction
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During postnatal development, the hippocampal signaling cascades for long-term potentiation (LTP) induction switch (Yasuda et al., 2003 ) and the NMDA receptor (NMDAR) 2B (NR2B)/NMDAR 2A (NR2A) ratio decreases (Sans et al., 2000). At postnatal day 28 (P28), rodents express comparable amounts of these NR2 subunits (Sans et al., 2000), but the specific signaling roles of the NMDAR subtypes are still unclear. Recent studies using NMDAR subtype blockade proposed that NR2A-type NMDARs induce LTP and NR2B-type NMDARs induce long-term depression (LTD) (Liu et al., 2004; Massey et al., 2004). In contrast, a role of NR2B-type NMDARs in LTP has been demonstrated in genetically modified mice, with overexpression of NR2B or upregulated NR2B expression leading to enhanced LTP (Tang et al., 1999; Wong et al., 2002) and activation of NR2B-type NMDARs restoring LTP in mice with absent or impaired NR2A signaling (Kiyama et al., 1998; Köhr et al., 2003). Because compensatory mechanisms might have contributed to the results from genetically modified mice, we have now used NR2-specific antagonists to test the contribution of NMDAR subtypes to hippocampal LTP.
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Materials and Methods
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Recombinant receptors. Human embryonic kidney 293 (HEK293) cells were cotransfected with plasmids encoding NR1-1a, NR2A, or NR2B and green fluorescent protein (GFP) (Chen and Okayama, 1987). Forty-eight hours after transfection, cells were continuously perfused with the following (in mM): 135 NaCl, 5.4 KCl, 1.8 CaCl2, and 5 HEPES, pH 7.3. Patch electrodes (4-7 M ) were filled with the following (in mM): 140 CsCl, 2 MgATP, 10 EGTA, and 10 HEPES, pH 7.3, 290-305 mOsm. GFP-labeled cells were lifted from the coverslip, and whole-cell currents were activated at -60 mV in the presence of 50 µM glycine by fast applying 1 mM glutamate from a Piezo-driven double-barreled pipette using an EPC-9 amplifier (HEKA Elektronik, Lambrecht, Germany), either in the absence or the presence of NR2 antagonists [e.g., the NR2A-specific antagonist NVP-AAM077 (NVP) (Novartis Pharma, Basel, Switzerland) or the NR2B-specific antagonist CP-101,606 (CP) (Pfizer, Groton, CT)]. NVP-AAM077 [(1R,1'S)-PEAQX], the full name of which is [(R)-[(S)-1-(4-bromo-phenyl)-ethylamino]-(2,3-dioxo-1,2,3,4-tetrahydroquinoxalin-5-yl)-methyl]-phosphonic acid, has first been reported as (1RS, 1'S)-diastereoisomeric mixture in the study by Auberson et al. (2002). More recent studies (Feng et al., 2004; Liu et al., 2004), including ours, used NVP-AAM077. Some reports (Feng et al., 2004) refer to NVP-AAM077 as PEAQX.
Whole-cell experiments. All experimental procedures were in accordance with the animal welfare guidelines of the Max-Planck-Society. The brain was removed from deeply anesthetized P28 mice (halothane), and transverse hippocampal slices (250 µm) were prepared and incubated for 30 min at 35°C in artificial CSF (ACSF) containing the following (in mM): 125 NaCl, 25 NaHCO3, 2.5 KCl, 1.25 NaH2PO4, 1 MgCl2, 25 D-glucose, and 2 CaCl2, bubbled with 95% O2/5% CO2, pH 7.4. Patch pipettes were pulled from borosilicate glass capillaries and had resistances of 4-6 M when filled with the following (in mM): 125 Cs-gluconate, 20 CsCl, 10 NaCl, 10 HEPES, 0.2 EGTA, 4 MgATP, and 0.3 Na3GTP, pH 7.3, 290-305 mOsm. Liquid junction potentials were not corrected. Series resistances (15-30 M) and input resistances (100-300 M) were continuously monitored by measuring the peak and steady-state currents in response to hyperpolarizing pulses (-5 mV; 20 ms). All patch experiments were performed at room temperature.
EPSCs were activated by stimulating the Schaffer collaterals  150 µm distant from the CA1 cell body with a glass electrode filled with 1 M NaCl. NMDA EPSCs were recorded at -40 and +40 mV in ACSF (see above) containing 10 µM bicuculline methiodide (BMI), 5 µM 2,3-dihydroxy-6-nitro-7-sulfonyl-benzo[f]quino-xaline-7-sulfonamide (NBQX), and 10 µM glycine. For LTP recordings, patch pipettes were filled with the following (in mM): 120 Cs-gluconate, 10 CsCl, 10 HEPES, 8 NaCl, 0.2 EGTA, 2 MgATP, 0.3 Na3GTP, and 10 phosphocreatine. Composite EPSCs were evoked in solutions containing the following (in mM): 124 NaCl, 26 NaHCO3, 1.25 NaH2PO4, 2.5 KCl, 4 CaCl2, 4 MgSO4, 10 D-glucose, 0.010 glycine, and 0.010 BMI, pH 7.3, 290-305 mOsm. In some experiments, D-2-amino-5-phosphonopentanoic acid (D-AP-5; Tocris Cookson, Bristol, UK), NVP-AAM077, or CP-101,606 was present. LTP was induced by pairing low-frequency stimulation (LFS) (120 pulses; 0.7 Hz) in the test (stratum radiatum) but not in the control pathway (stratum oriens) with postsynaptic depolarization to 0 mV for 3 min (LFS pairing). Six single EPSCs were averaged and normalized to the averaged responses obtained 5 min before LTP induction. EPSC potentiation was assessed 30 min after induction.
Extracellular field experiments. P28 mice were killed with desflurane. Transverse slices (400 µm) from the middle portion of each hippocampus were cut with a vibroslicer in the following ACSF (in mM): 124 NaCl, 2 KCl, 1.25 KH2PO4, 2 MgSO4, 2 CaCl2, 26 NaHCO3, and 12 D-glucose; 4°C, bubbled with 95% O2/5% CO2, pH 7.4. Slices were placed in an interface chamber at 28-32°C and were perfused with ACSF, which in some experiments contained NVP-AAM077. Orthodromic synaptic stimulation was delivered alternately through two tungsten electrodes, one in the stratum radiatum and the other in the stratum oriens. Extracellular responses were monitored in the corresponding layers by two glass electrodes filled with ACSF. Assessment of synaptic efficacy and tetanization procedures were as described previously (Köhr et al., 2003).
All data were pooled across animals and are presented as mean ± SEM. Statistical significance was evaluated using a two-tailed Student's t test.
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Figure 1. Effects of NVP-AAM077 on recombinant and synaptic NMDARs and on LTP elicited by tetanization. A, Example traces of glutamate-evoked currents in HEK293 cells expressing NR1/NR2A or NR1/NR2B receptors in the absence (black) and presence (gray) of NVP. Summary of results using different NVP concentrations (gray; *p < 0.0005) or 10 µM CP-101,606 (striped) is shown. B, NMDA EPSC traces recorded in slices from P28 NR2A knock-out mice in 10 µM bicuculline, 5 µM NBQX, and 10 µM glycine at -40 and +40 mV in the absence (black) and presence (gray) of 50 nM NVP. Summary of results using different NVP concentrations (gray; *p = 0.007 and **p = 0.0004) is shown. C, Single tetanization (100 Hz; 1 s) induced LTP in hippocampal slices from P28 wild-type mice (open circles) in the presence of 50 and 400 nM NVP (*p < 0.001 and *p = 0.003, filled circles and diamonds, respectively). Repeated tetanizations (4 times with 5 min intervals) in the presence of 50 nM NVP (filled squares) increased LTP to levels obtained without the blocker (open squares). A representative control pathway is shown (open triangles). The arrow indicates the time of both the first and the fourth tetanization. Data are mean ± SEM.
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Results
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Effects of the NR2A-specific antagonist NVP-AAM077 on recombinant and synaptic NR2B-type NMDARs
The NR2A-specific antagonist NVP-AAM077 (for details of the former name PEAQX, see Materials and Methods) has a 130-fold preference for recombinant human NR1/NR2A over NR1/NR2B receptors expressed in oocytes (Auberson et al., 2002), whereas subtype selectivity is only 13-fold for recombinant rodent NMDARs (Feng et al., 2004). Indeed, NVP strongly reduced glutamate-evoked peak currents in HEK293 cells expressing rodent NR1/NR2A receptors but also reduced currents mediated by rodent NR1/NR2B receptors at all concentrations tested (Fig. 1A) [50 nM, 77 ± 3% (n = 12) vs 27 ± 3% (n = 10) and 77 ± 3% (n = 12), p < 0.0001; 100 nM, 91 ± 1% (n = 12) vs 37 ± 6% (n = 7), p < 0.0005; 400 nM, 97 ± 1% (n = 3) vs 67 ± 3% (n = 4), p < 0.005]. In contrast to NVP, the NR2B-specific antagonist CP, which is more potent than ifenprodil (Mott et al., 1998), is >1000-fold more selective for NR1/NR2B than NR1/NR2A receptors (Nagy et al., 2004). CP at 10 µM reduced NR1/NR2B but not NR1/NR2A receptor currents (Fig. 1A) (68 ± 4%, n = 4 vs 5 ± 2%, n = 4; p = 0.0002). We next recorded pharmacologically isolated NMDA EPSCs from CA1 pyramidal cells in acute slices from 4-week-old NR2A-/- mice (Sakimura et al., 1995). NR2B-mediated NMDA EPSCs were similarly reduced by NVP at -40 and +40 mV, respectively (Fig. 1B) [50 nM NVP (n = 8), 25 ± 6 and 19 ± 4%, p = 0.25; 400 nM NVP, 56 ± 2% (n = 5) and 46 ± 3%, p < 0.06], in agreement with results from recombinant NR1/NR2B receptors.
Thus, NVP is not an NR2A-type selective antagonist but preferentially antagonizes NR2A-type NMDARs when used at 50 nM.
Tetanic stimulation
A single tetanization in the hippocampal CA1 region (100 Hz, 1 s; wild type; P28) (see Materials and Methods) elicited robust LTP of the field EPSP (Fig. 1C) (1.37 ± 0.07; n = 22). Consistent with the proposed role of NR2A-type NMDARs, 50 nM NVP reduced but failed to block LTP (1.17 ± 0.04; n = 19; p = 0.017). Increasing the NVP concentration to 400 nM, which should completely prevent the activation of NR2A-type NMDARs based on the above results on recombinant NMDARs, did not further reduce LTP (Fig. 1C) (1.15 ± 0.07; n = 9). Thus, LTP can be induced by tetanic stimulation in the presence of 400 nM NVP via NR2B-type NMDARs, which was not observed by Liu et al. (2004). Our results are consistent with studies demonstrating that NR2B-type NMDARs also induced LTP in mice with absent or impaired NR2A signaling, most efficiently after repeated tetanizations (Kiyama et al., 1998; Köhr et al., 2003). Indeed, we found additional proof for the role of NR2B in LTP induction in wild type when NR2A-type NMDARs were blocked by 50 nM NVP, because four tetanic stimulations increased LTP (Fig. 1C) (1.46 ± 0.08; n = 14; p = 0.001) to the level obtained without NVP (1.43 ± 0.07; n = 16; p = 0.88).
Thus, NR2B-type NMDARs induce LTP when NR2A-type signaling is genetically (Kiyama et al., 1998; Köhr et al., 2003) or pharmacologically impaired.
Low-frequency stimulation
In whole-cell experiments from CA1 neurons (P28), we paired low-frequency stimulation with postsynaptic depolarization (LFS pairing, 0.7 Hz, 3 min; 0 mV) (see Materials and Methods). This protocol increased EPSCs (Fig. 2A) (2.05 ± 0.2; n = 12), even in the presence of 50 nM NVP (Fig. 2C) (2.12 ± 0.2; n = 10; p = 0.8), when NMDA EPSCs were reduced by 40% (Fig. 2B) [-40 mV, 39 ± 4% (n = 11); +40 mV, 35 ± 3% (n = 11)]. LTP was still induced in the presence of 100 nM NVP (1.8 ± 0.5; n = 3; p = 0.9) but was abolished at 200 and 400 nM NVP (Fig. 2C) (1.2 ± 0.3, n = 3; 1.1 ± 0.1, n = 4). Thus, LTP could not be induced in the presence of 400 nM NVP by LFS pairing, contrasting the results obtained by tetanic stimulation (see above). Note that the reduction of the NMDA EPSCs was doubled by 400 nM NVP compared with 50 nM NVP (Fig. 2B) [-40 mV, 81 ± 1% (n = 7); +40 mV, 65 ± 3% (n = 5)]. Importantly, after preferred blockade of NR2A-type NMDARs (50 nM NVP), LTP is induced by NR2B-type NMDARs using LFS pairing. This contrasts with the observation of Liu et al. (2004), who used a comparable LFS pairing protocol (2 Hz, 1.7 min; -5 mV) and 400 nM NVP, which in their hands reduced NMDA EPSCs by 53% and converted LTP into LTD.
To further substantiate that both NMDAR subtypes are capable of inducing LTP, we used 10 µM CP, which like 50 nM NVP reduced NMDA EPSCs at +40 mV by 38 ± 8% (Fig. 2B)(n = 11) (Steigerwald et al., 2000). LTP was not affected by CP (Fig. 2C) (1.7 ± 0.03; n = 6; p = 0.15), suggesting that LFS pairing induces LTP regardless of which NR2 subtype is blocked. Similarly, even D-AP-5, a nonselective NMDAR antagonist, at a concentration reducing NMDA EPSCs by 40% (Fig. 2B)[-40 mV, 43 ± 4% (n = 6); +40 mV, 41 ± 4% (n = 6)] (Nishiyama et al., 2000), failed to impair LTP (Fig. 2C) (2.1 ± 0.3; n = 6; p = 1.0).
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Figure 2. Comparison of the effects of different NMDAR antagonists on synaptic NMDA EPSCs and on LTP elicited by LFS pairing. A, EPSCs were evoked at -70 mV in 10 µM BMI and 10 µM glycine in slices from P28 wild-type mice. LFS pairing (bar) increased EPSCs of the test (open circles) but not of the control pathway (open triangles). Representative EPSCs for test are labeled with 1 and 2 and, for control pathway, with a and b. B, NMDA EPSC traces recorded in slices from P28 wild-type mice at -40 and +40 mV in the absence (black) and presence (gray) of 50 nM NVP. Summary of results at +40 mV using NVP (gray; *p < 0.0001), CP (hatched), or D-AP-5 (cross-hatched) is shown. C, Changes in EPSC amplitude after LFS pairing in the absence (white) and presence (B) of different NMDAR antagonists (*p = 0.002). Data are mean ± SEM.
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Therefore, LFS pairing in the presence of NR2 subtype-specific antagonists fails to reveal a preferred NR2 subtype for LTP induction.
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Discussion
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Using a pharmacological approach, we demonstrate that LTP induction at CA3-to-CA1 synapses by different induction protocols does not require a particular NMDAR subtype. A 40% blockade of synaptic NMDARs by three antagonists (two preferentially blocking NR2A- or NR2B-type NMDARs and one nonselectively blocking both subtypes) did not impair LFS pairing-triggered LTP. Furthermore, LTP induction by repeated tetanic stimulation was not prevented by the NR2A- and NR2B-preferring antagonists (Köhr et al., 2003; our observations).
Our findings that NR2B-type NMDARs can induce LTP are consistent with those obtained in genetically modified mice, in which NR2B expression was upregulated (Tang et al., 1999; Wong et al., 2002) or NR2A signaling was impaired (Kiyama et al., 1998; Köhr et al., 2003). This role for NR2B in LTP induction was not observed by Liu et al. (2004), who failed to induce LTP in the presence of 400 nM NVP by either tetanization or LFS pairing, although the NMDA EPSCs were only reduced by 53%. In our study, NMDA EPSCs that were reduced to even higher extents by 400 nM NVP still allowed LTP induction by tetanization in field recordings. In contrast, LTP could not be induced by LFS pairing in the presence of 400 nM NVP. Our contrasting results are not explained by the two recording configurations (field vs whole cell) but can be explained by the distinct induction protocols used. In a former study, which investigated LTP in CA1 neurons of genetically modified mice expressing NMDARs with reduced Ca2+ permeability (by 65%) (Pawlak et al., 2005), we also observed that LTP was not induced by LFS pairing but by tetanic stimulation. Therefore, the frequency-dependent impairment of hippocampal LTP could be induced genetically and pharmacologically by NVP. This frequency-dependent impairment may be explained by NMDAR-mediated responses reaching threshold, probably because of summation during tetanization but not during LFS pairing (Pawlak et al., 2005).
A role for NR2B in LTP induction was not observed in the adult cortex (Massey et al., 2004). Different from hippocampal synapses (Steigerwald et al., 2000), CP did not reduce the NMDA EPSCs at cortical or cerebellar synapses in rodents older than P14 (Stocca and Vicini, 1998; Rossi et al., 2002), which may preclude NR2B-type NMDARs from inducing LTP in these but not in CA3-to-CA1 synapses. A partial restriction of the NR2B pathway at hippocampal synapses became evident during short tetanizations, which gave the NR2A-subtype advantage over NR2B, perhaps reflecting the faster signaling properties of NR2A than of NR2B (Erreger et al., 2005). Therefore, the relative contribution of the NR2 subtypes in LTP induction in CA1 neurons could be determined by the frequency pattern of synaptic stimulation, which might also influence the direction of the plasticity response. This is illustrated by a 40% NMDAR blockade, which did not reverse LTP induced by LFS pairing (0.7 Hz in our study) but reversed LTP after 20 Hz stimulation (Cummings et al., 1996) or spike timing (Nishiyama et al., 2000).
In summary, our results do not support the model that only NR2A-type NMDARs are responsible for LTP induction (Collingridge et al., 2004). Moreover, previous and unpublished observations (Hendricson et al., 2002) (W. Morishita and R. C. Malenka, personal communication) also cast doubt on the proposed preponderance of NR2B in LTD induction (Collingridge et al., 2004). Thus, rather than resulting from exclusive roles of NMDAR subtypes, the synaptic plasticity response appears to be directed by the pattern of synaptic activation, which recruits the major NMDAR subtypes to variable extents and triggers distinct signaling cascades.
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Footnotes
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Received May 12, 2005;
revised June 15, 2005;
accepted June 16, 2005.
We thank Dr. M. Mishina (University Tokyo) for providing GluR 1 (NR2A) KO mice, Dr. Y. P. Auberson (Novartis Pharma AG, Basel, Switzerland) for discussion and the gift of NVP-AAM077, and Pfizer (Groton, CT) for the gift of CP-101,606. We also thank Dr. F. N. Single, J. Kling, and Liya Pan for experimental help.
Correspondence should be addressed to Dr. Georg Köhr, Department of Molecular Neurobiology, Max-Planck-Institute for Medical Research, Jahnstrasse 29, D-69120 Heidelberg, Germany. E-mail: kohr{at}mpimf-heidelberg.mpg.de.
Copyright © 2005 Society for Neuroscience 0270-6474/05/256907-04$15.00/0
* S.B. and P.P contributed equally to this work. 
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Top
Abstract
Introduction
