The Journal of Neuroscience, July 27, 2005, ():
Essential Role for Survivin in Early Brain Development
J. Neurosci. Jiang et al.
25: 6962
Supplemental data
Files in this Data Supplement:
- supplemental material
- supplemental material
- supplemental material
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Supplemental Figure 1: Expression of cre recombinase in nestin-cre;rosalox/+ mice
a). X-gal stained whole mounts of nestin-cre;cre-reporter mice at various developmental time points showing b-galactosidase activity (blue) in the developing brain, eye, and spinal cord.
b). Sections of E11.5 nestin-cre;cre-reporter brain showing b-galactosidase activity (blue). The arrows indicate regions of the brain negative for b-galactosidase. OR, optic recess; CI, choroid invagination; 4th, 4th ventricle; 3rd, 3rd ventricle; LV, lateral ventricle; TG, trigeminal ganglia; A, anterior; P, posterior. Bars = 250 µm.
- supplemental material
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Supplemental Figure 2: Genotyping analysis of microdissected tissue
a). Schematic diagram showing the survivinlox/lox genetic locus, including exons 1-4, and the DNA primers used to determine the presence or absence of survivin.
b). DNA gel electrophoresis showing PCR fragments detected using a combination of primers including Adv17, Adv25, and Adv28. Sections were visualized under microscopy and tissues dissected using a Zeiss Microlaser System, as described in Materials and Methods. Tissues were obtained from embryos at various developmental stages (lanes 1-3, E12; lanes 4-5, E14.5; lanes 6-8, E17.5).
