The Journal of Neuroscience, July 27, 2005, ():
Pax6 Is Required for Making Specific Subpopulations of Granule and Periglomerular Neurons in the Olfactory Bulb
J. Neurosci. Kohwi et al.
25: 6997
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplementary Figure 1: Proportion of Pax6+ neurons generated in the adult brain. BrdU/Pax6 double-labeled cells among total BrdU+ cells were counted in the GCL and glomerular layer 45days after a single BrdU injection. For granule cells n=1616 from 3 mice; periglomerular cells n=509 from 3 mice. Error bars were calculated as the standard error of the mean.
- supplemental material
-
Supplementary Figure 2: Proportion of TH+ PGCs generated in the adult brain. BrdU/TH double-labeled cells among total BrdU+ cells were counted in the glomerular layer 45days after a single BrdU injection. n=571 cells from 3 mice were counted. a~b shows examples of TH-BrdU double-labeled PGCs. c, quantification of TH+ PGCs generated in adult brain as compared to the proportion of Pax6+ PGCs generated in the adult brain. Error bars were calculated as the standard error of the mean.
- supplemental material
-
Supplementary Figure 3: Pax6Sey/Sey graft-derived cells in the deep GCL have branched dendrites and dendritic spines typical of mature OB GCs. a, the dendritic branch shown is in the deep external plexiform layer from a Pax6Sey/Sey graft-derived cell in the deep GCL. b, the Pax6Sey/Sey graft-derived neurons also have dendritic spines. Scale bar for a = 20?m, scale bar for b = 10?m.
- supplemental material
-
Supplementary Figure 4: Er81 protein expression in the adult OB. Schematic shows area depicted in image below. Note how Er81 expression in the GCL predominates in the superficial layer. GCL, granule cell layer; EPL, external plexiform layer.
