The Journal of Neuroscience, July 27, 2005, ():
Pattern-Dependent Role of NMDA Receptors in Action Potential Generation: Consequences on Extracellular Signal-Regulated Kinase Activation
J. Neurosci. Zhao et al.
25: 7032
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1. Bath application of CPP reduces the number of action potentials generated in response to 5 Hz and 100 Hz synaptic stimulation. (A) Recordings from a representative cell in response to stimulation at 5 Hz prior to, during, and after washout of CPP (20 |*mu*|M). Of thirty traces recorded in each condition, only eight are shown superimposed for clarity of the figure. (B) Recordings from a representative cell in response to 100 Hz stimulation in the presence of CPP and after washout. Compiled data from the same experiments are shown to the right of traces from each frequency. In the 100 Hz case, a 5 Hz test stimulation was used to assess wash-in of the drug, and the number of action potentials in the presence of drug compared with that in the absence of drug (washout). Shown are means +/- SEMs from 11 cells (5 Hz) and 11 cells (100 Hz).
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Supplementary Figure 2. CPP, at a concentration that blocks action potential generation, has no effect on AMPA currents. Bath application of 20 |*mu*|M CPP has no effect on AMPA- mediated EPSCs.(A) Data are means +/- SEMs from 14 cells. Representative recordings of EPSCs with and without 20 |*mu*|M CPP are shown superimposed . (B) Shown are the averages of 10 traces.
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Supplementary Figure 3. Intracellular MK801 prevents action potential generation induced with 5 Hz stimulation. Shown are traces from recordings immediately after break-in (A) and 5 minutes after break-in (B) when 20 |*mu*|M MK801 was included in the internal solution of the patch pipette.
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Supplementary Figure 4. Increasing stimulus intensity fails to fully compensate for the effects of NMDAR antagonists. Bath application of 50 |*mu*|M APV reduces the number of action potentials generated with 5 Hz stimulation. The number of action potentials induced with 30 stimuli are plotted against stimulation intensity. Shown are means +/- SEMs from 6-20 cells.
