The Journal of Neuroscience, August 3, 2005, ():
Decreased Sensory Stimulation Reduces Behavioral Responding, Retards Development, and Alters Neuronal Connectivity in Caenorhabditis elegans
J. Neurosci. Rose et al.
25: 7159
Supplemental data
Files in this Data Supplement:
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Supplemental Figure. Effects of isolation on response to tap in 5 different strains of worms, measured as the mean of the response magnitude divided by worm length, and worm length in daf-2(e1371), a predicted tyrosine receptor kinase that is the C. elegans insulin/IGF receptor (Kimura et. al., 1997; a, N=66, t(64) = 2.24, p=0.028; b, t(65) = 2.34, p= 0.022), cat-2(e1112), a mutation in tyrosine hydroxylase which is then deficient in dopamine (Sulston et. al., 1975; c, N=35, t(33) = 2.09, p=0.044; d, t(33) = 2.47, p = 0.019), avr-14(da1371), a glutamate gated chloride channel (Dent et. al., 2000; e, N=57, t(55) = 3.03, p=0.004; f, t(57) = 3.80, p=0.0004), nmr-1(ak4), an NMDA-type glutamate receptor (Brockie et. al., 2001; g, N=49, t(47)=2.25, p=0.029; h, t(47) = 2.14, p=0.037) and npr-1(n1353) a neuropeptide Y-type receptor (de Bono et. al., 1998; i, N=45, t(43) 2.11, p=0.04; j, N=92, t(90) 2.24, p=0.027; (in the initial test with 45 npr-1 worms the worm length was not significant but was trending in the same way as wild-type worms, therefore an additional 47 worms were tested to confirm the effect of isolation on worm length). * = p<0.05.
Supplementary References
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de Bono, M. & Bargmann, C.I. (1998) Natural variation in a neuropeptide Y receptor homolog modifies social behavior and food response in C. elegans. Cell 94, 679-689.
Dent, J.A., Smith, M.M., Vassilatis, D.K., & Avery, L. (2000). The genetics of ivermectin resistance in Caenorhabditis elegans. PNAS 97, 2674-2679.
Kimura, K.D., Tissenbaum, H.A., Lui, Y. and Ruvkun, G. (1997). daf-2, an insulin receptor- like gene that regulates longevity and diapause in Caenorhabditis elegans. Science 277, 942–946
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