The Journal of Neuroscience, August 3, 2005, ():
The Caudal Migratory Stream: A Novel Migratory Stream of Interneurons Derived from the Caudal Ganglionic Eminence in the Developing Mouse Forebrain
J. Neurosci. Yozu et al.
25: 7268
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1
Focal electroporation into the telencephalic hemisphere. The telencephalic hemispheres were placed between a platinumplate electrode (positive pole) and a tungsten needle electrode (negative pole) in PBS. Plasmid solution was injected into the MGE or CGE with a glass micropipette, and electronic pulses were generated.
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Supplementary Figure 2
Time-lapse imaging of the tangentially migrating cells derived from the MGE. (A, B) Coronal slices were prepared at 40 hours in vitro after focal electroporation into the MGE of E13.5 telencephalic hemispheres. The asterisk shows the place of electroporation. (B) Time-lapse images of migrating cells from the boxed area in (A). A number of MGE cells migrated tangentially from the MGE to the cortex. The solid arrowhead (0-60 min), open arrowhead (45-105 min) and the solid arrow (30-105 min) point to three tangentially migrating cells (For a time-lapse movie, see Supplementary Movie 1 online). Scale bars, (A) 200 µm, (B) 50 µm.
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Supplementary Figure 3
The CGE-derived cells reached the MZ of the posterior cerebral cortex. (A-H) DsRedEx/CAG was electroporated into the CGE of E13.5 telencephalic hemispheres and the hemispheres were cultured for 40 hours. Lateral (A-D) and caudal (E-H) images (A, E: bright field; B-D, F-H: fluorescent) of whole-mount telencephalic hemispheres show the CGE-derived cells (red) on the surface of the posterior cerebral cortex. (A, B) Anterior is to the right and posterior is to the left. (C) Higher magnification of the boxed area in (B). (D) Higher magnification of the boxed areas in (C). (G) Higher magnification of the boxed area in (F). (H) Higher magnification of the boxed area in (G). Scale bars, (A, B, E, F) 400 µm, (C, G) 200 µm, (D, H) 60 µm.
- supplemental material
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Supplementary Movie 1
Time-lapse imaging of the tangentially migrating cells derived from the MGE. A number of MGE cells migrated tangentially from the MGE to the cortex (See Supplementary Figure 2 for details).
- supplemental material
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Supplementary Movie 2
Time-lapse imaging of the CMS. A significant number of the CGE-derived cells exhibited a clear migratory stream from the site of electroporation in the CGE to the hippocampus (See Figure 3 for details). The morphological features and migratory behavior of migrating CGE-derived cells resembled those of 'branching cells' (Nadarajah et al. 2003) in that they were highly motile in the formation and retraction of their processes.
- supplemental material
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Supplementary Movie 3
Time-lapse imaging of the CGE-derived cells within the hippocampus. The CGE-derived cells in the migratory stream changed their direction and moved toward the MZ (See Figure 3 for details).
