The Journal of Neuroscience, August 10, 2005, ():
Olig2 Directs Astrocyte and Oligodendrocyte Formation in Postnatal Subventricular Zone Cells
J. Neurosci. Marshall et al.
25: 7289
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplemental Figure S1: Specificity of Olig2 antibody. Immunohistochemical analysis of spinal cord sections taken from e10.5 Olig2+/- control and Olig2-/- mutant mouse embryos.
- supplemental material
-
Supplemental Figure 2: Model of SVZ Cell Specification within the Neonatal Forebrain
Olig2 represses a neuronal phenotype in SVZ progenitors, allowing or promoting the emergence of glioblasts. Previous in vivo clonal analyses suggest that while a percentage of these glial precursors will remain uncommitted for a period of time, others will become committed to an astrocyte or oligodendrocyte-specific lineage almost immediately following their emigration from the SVZ.
SVZ progenitors negative for Olig2 become specified as interneuronal precursors, ultimately differentiating into olfactory interneurons. Transcription factors directing SVZ progenitors to a neuronal fate, potential targets for Olig2 repressor activity, are yet unknown.
Olig2 expression is downregulated in maturing astrocytes but maintained in oligodendrocytes. While constitutive Olig2 expression appears to promote oligodendrocyte differentiation, perhaps even prematurely, it may alternatively inhibit the final stages of astrocyte maturation.
In summary, Olig2 directs a pan-glial versus neuronal cell fate decision in SVZ progenitors. Furthermore, it may serve two different roles in maturing glial cells by promoting oligodendrocyte differentiation and regulating of astrocyte maturation.
