The Journal of Neuroscience, August 17, 2005, ():
Feedforward Inhibitory Control of Sensory Information in Higher-Order Thalamic Nuclei
J. Neurosci. Lavallée et al.
25: 7489
Supplemental data
Files in this Data Supplement:
- supplemental material
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Fig. S1 Immediate inactivation of the cerebral cortex by topical application of silver nitrate on the pia. A, electrocorticogram recorded in layer 4 of the barrel field (barrel B2, depth: 750 µm) under ketamine anesthesia. Note the negative field potentials elicited by air jet stimulation of the vibrissae. B, electrocorticogram recorded 20 sec after application of a small crystal of silver nitrate on the pia. The trace flattened almost immediately and evoked potentials disappeared. The extent of the lesion is shown in C and D after Nissl staining and cytochrome oxidase histochemistry, respectively.
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Fig. S2 Spontaneous activity and whisker-evoked responses in ZI cells under urethane anesthesia. A, the low discharge rate of this unit (2.5 Hz) is representative of that observed in ZId and ZIv under urethane anesthesia (ratemeter display with 1 s bin width). The PSTH in B shows grouped responses of 10 ZI cells to whisker deflection (mean response onset latency: 4.75 ± 1.32 ms).
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Fig. S3 Responses of a VPM neuron to whisker deflection. A, intracellular recording obtained under light ketamine anesthesia in an intact rat (no lesion). Note the smooth aspect of the membrane potential at rest (compared to the noisy resting potential of Po cells; Figure 3B), and also note that responses consist of EPSPs followed by IPSPs. Traces in B show responses with an expanded time scale, and the PSTH in C compiles 20 responses of the same unit.
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Fig. S4 Inhibition precedes excitation in Po cells when ZI is intact. Intracellular recordings from a Po cell under urethane anesthesia; ZI was intact and the barrel cortex was lesioned. Note that, as in Fig. 5D, whisker deflection first evoked an IPSP followed by an EPSP. This contrasts with responses evoked in VPM cells, in which excitation precedes inhibition (see Fig. S3).
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Fig. S5 SpVi afferents innervate Po and are immunoreactive for vGlut2. A, low power light micrograph of a horizontal brainstem section showing a BDA injection in the rostral part of SpVi. The anterograde tracer labels a band of fibers and terminals restricted to the calbindin-positive region of Po, and avoids the calbindin-negative VPM, as shown in a coronal section (B) doubly stained for BDA (black) and calbindin (brown). Some of the calbindin-positive cells are labeled by arrowheads. C, high power light micrographs of fluorescent staining for BDA (green) and vGlut2 (red) in Po following BDA injection into the SpVi. Note that all BDA-positive terminals (arrows) contain vGlut2. Scales: A, 1mm; B, 100 µm; C, 10 µm). Abbreviations: 7th, tractus of the facial nerve; sp5, spinal trigeminal tract; SpVc, caudalis division of the spinal trigeminal complex; SpVo, oralis division of the spinal trigeminal complex.
