The Journal of Neuroscience, September 7, 2005, ():
Recurrent Mossy Fibers Establish Aberrant Kainate Receptor-Operated Synapses on Granule Cells from Epileptic Rats
J. Neurosci. Epsztein et al.
25: 8229
Supplemental data
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Fig. S1 Granule cells from 4-5 d post-status epilepticus rats do not generate EPSCKA even in conditions of increased spontaneous activity
(A) Spontaneous EPSCs recordings in the presence of bicuculline (10 然), and D-APV (40 然) in a granule cell from a 4 d post-status epilepticus rat. Note that application of 4-AP (100 然) strongly increases the frequency of EPSCs, that are all mediated by AMPARs since they are fully abolished by 1 然 NBQX.
(B) Histogram plot of the number of spontaneous EPSCs versus time before and during bath application of 100 然 4-AP in the granule cell shown in (A). The frequency of spontaneous EPSC is strongly increased during 4-AP application. Subsequent application of 1 然 NBQX fully abolished all the spontaneous EPSCs.
(C) Bar graphs of the mean frequency of spontaneous EPSCs (recorded in the presence of bicuculline and D-APV, n=5) in control granule cells, and in 4-5 d post-status epilepticus rats in the absence (Post status) and in the presence of 4-AP (100 然). Note that the frequency of spontaneous events recorded in granule cells from 4-5 d post-status epilepticus rats is not significantly different from that recorded in control granule cells (n=5, p>0.05) and is significantly increased in the presence of 4-AP (n=5, *p<0.05).
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Fig. S2 Focal stimulation in the hilus evokes only EPSCAMPA in granule cells from epileptic rats
(A) GluR2/3 staining of the hilar mossy cells in the hilus (H) of control (upper) and epileptic (lower) rats. Though the number of hilar mossy cells is decreased, immunoreactive cells (arrows) remain in the chronic epileptic rat. No major change was observed in the granule cell and pyramidal cell layers. Abbreviations: g= granule cell layer; Bars=100 痠.
(B) Drawing of a hippocampal slice depicting the position of the recording pipette and the locus for focal application (puff, 50 ms duration) of 30 然 KA.
(C) Puff application of KA (arrow) induces an increase in the frequency of spontaneous EPSCs in a distant recorded granule cell from an epileptic rat.
(D) Enlargement of the recording shown in C enable the identification of spontaneous EPSCAMPA (black dot) and EPSCKA (star) before (Di, Control period) and after (Dii, Stimulation) the distant application of KA. Only the frequency of EPSCAMPA but not that of EPSCKA is enhanced. This effect is reversible (Diii, Wash). Traces are consecutives in Di, Dii and Diii and correspond to i, ii and the wash period indicated in c.
(E) Bar graphs of the mean frequency of spontaneous EPSCAMPA and EPSCKA recorded before (Cont.) and during the stimulation period (Stim., 60 sec) in granule cells from epileptic rats. The frequency of EPSCAMPA is significantlyincreased (n=7 stimulations in 7 cells; *p<0.05) but not the frequency of EPSCKA (p>0.05).
