The Journal of Neuroscience, September 21, 2005, ():
Independent Circadian Oscillations of Period1 in Specific Brain Areas In Vivo and In Vitro
J. Neurosci. Abraham et al.
25: 8620
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Fig. 1. SCN-lesions abolished circadian locomotor patterns, but not rhythms in Per1::luc activity in the OB. A-D: Representative double-plotted actograms showing wheel-running activity rhythms of an intact rat in a dark-light cycle (A, lights on from 19:00 to 07:00) and of SCNX rats in constant darkness (B-D). Each vertical black bar indicates the percentile of wheel-running activity from that day. The red asterices denote the time of OB window surgery, two days prior to the onset of OB imaging. Chi-squared periodograms in the lower right corners show the dominant periods within the last 10 days of the respective activity recordings. Yellow bars indicate the light phase. While the wheel-running activity of the intact rat was clearly entrained to the dark-light cycle with a period of 24 h (A), SCNX rats in constant darkness exhibited arrhythmic activity patterns with no significant circadian periods (B-D). E-H: To the right of each actogram is the corresponding normalized Per1::luc bioluminescence trace obtained by OB in vivoimaging. The OB of the intact rat showed a significant circadian rhythm of Per1::luc with a peak during the early subjective night (COSOPT, ?=0.19, p=0.011; E). SCN ablation did not abolish rhythms of Per1::luc in the OB. We show representative traces of two rhythmic OB (F: ?=0.27, p=0.007; G: ?=0.12, p=0.01) and one arrhythmic OB (H: ?= 0.2, p=0.067) from SCNX rats. Horizontal grey and black bars indicate the previous light and dark times. B-C and F-G are recordings from rats B and C in Fig.1.
- supplemental material
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Supplemental Fig. 2. Representative in vitro recordings of Per1::luc activity from different brain regions of sham-operated and SCNX rats killed at CT 11. While the SCN of sham-lesioned rats continued to oscillate for the duration of the recording, the PI and VOLT displayed damped circadian rhythms with an average of 1 (PI, n =4; 1.0±0.0 SEM) or 3 (VOLT, n=3; 3.0±0.58 SEM) circadian cycles, respectively. In SCNX rats, these rhythms persisted with similar damping rates, phases, and periods. Bioluminescence was low and arrhythmic in explants containing the lesioned SCN.
