The Journal of Neuroscience, September 28, 2005, ():
Constitutive Secretion of Protease Nexin-1 by Glial Cells and Its Regulation by G-Protein-Coupled Receptors
J. Neurosci. Giau et al.
25: 8995
Supplemental data
Files in this Data Supplement:
- supplemental material
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Video 1. Budding of GFP/PN-1 vesicles from TGN.
C6 glioma cells expressing GFP/PN-1 were imaged every 2 seconds, 15 min post warm-up. The movie, focused on the TGN area, shows a vesicle (arrowhead) budding off and breaking off a discrete TGN domain.
- supplemental material
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Video 2. Fusion of GFP/PN-1 vesicles to plama membrane.
C6 glioma cells expressing GFP/PN-1 were imaged every second, 45 min post warm-up. The movie, focused at the cell periphery, shows a vesicle (arrowhead) that moved, stopped for several seconds and then disappeared.
- supplemental material
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Video 3. Transport of GFP/PN-1 vesicles from TGN to plasma membrane.
C6 glioma cells expressing GFP/PN-1 were imaged every 2 seconds, 30 min post warm-up. The movie shows numerous vesicles (arrowheads) budding from the TGN. The vesicle movement shows brief periods of rapid runs interrupted by resting periods. Thereafter, the vesicles continued either in the same direction, changed or reversed their direction.
- supplemental material
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Video 4. Microtubule-dependent transport of GFP/PN-1.
C6 glioma cells expressing GFP/PN-1 and ?-tubulin/DsRed were imaged every 2 seconds. The movie shows a single vesicle (arrow) moving along microtubule tracks (arrowhead) and switching between tracks.
- supplemental material
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Video 5. Cortical F-actin acts as a barrier that hinders GFP/PN-1 vesicle movement.
C6 glioma cells expressing GFP/PN-1 and EYFP/actin were imaged every 2 seconds. The movie, focused at the cell periphery, shows a single vesicle (arrows) moving towards cortical F-actin (arrowhead), sticking for several seconds and breaking off this barrier.
