The Journal of Neuroscience, October 5, 2005, ():
CHIP Suppresses Polyglutamine Aggregation and Toxicity In Vitro and In Vivo
J. Neurosci. Miller et al.
25: 9152
Supplemental data
Files in this Data Supplement:
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Supplemental Figure 1. Zebrafish embryos at 24 hours expressing polyQ proteins. (A) Low power brightfield images of live (left) and dead (right) 24 hour old zebrafish embryos. Embryos which lyse leaving only residual opaque tissue inside the chorion (right) are scored as dead. (B) High power brightfield and fluorescent illumination images of ~24 hr old zebrafish embryos expressing the indicated polyQ proteins. The uninjected and Q19-GFP expressing embryos have normal morphology and are fully transparent. The Q80-GFP expressing embryo is malformed and has prominent areas of brownish, opaque tissue (white arrow) indicative of cell death. The decreased eye pigmentation in the Q19 and Q80 injected embryos is due to a slight developmental delay caused by the injection procedure (B) Flat mount, high power image (630x) of a Q80-GFP expressing embryo. Shown are brightfield and fluorescent illumination of a region similar in appearance to the region indicated by the arrow in (A). Prominent staining of cells with acridine orange indicates apoptosis (red). Embryos were dechorionated and placed in 10 µg/ml acridine orange for 15 minutes (Furutani-Seiki et al., 1996). Embryos were then killed by incubating in ice cold tricaine and compressed under a glass coverslip for immediate imaging.
