The Journal of Neuroscience, October 5, 2005, ():
Sleep-Dependent Plasticity Requires Cortical Activity
J. Neurosci. Jha et al.
25: 9266
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1. Experimental design. Critical period cats were divided into 3 main (and 1 supplemental) groups to investigate the role of cortical activity during sleep in OD plasticity. In all groups, each experiment began with a 6 hour baseline sleep/wake period followed by a 6 hour period of monocular deprivation (MD) in normal illumination. All cats were then allowed to sleep ad lib in complete darkness for 6 hours. During this time VEH and LIDO cats had their visual cortices (ctx) infused with vehicle (VEH), or lidocaine (LIDO), respectively, while SHAM cats had equal amounts of lidocaine infused into the cerebro-spinal fluid just above visual cortex. The supplemental group of RESCUE cats were treated identically as the LIDO cats except that an additional 6 hour ad lib sleep period was provided without additional lidocaine infusion. At the end of the 6 hour sleep period (or Sleep 2 in RESCUE cats) all animals were immediately assayed for changes in ocular dominance (see Methods and Materials for details).
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Supplementary Figure 2. Lidocaine inactivation is widespread and reversible. Polar maps from representative hemisphere infused with lidocaine (0.3µl/minute). (a) vascular map, (b) pre-lidocaine infusion, (c) 30 minutes, (d) 1 hour, (e) 2 hours, (f) 3 hours, (g) 4 hours into lidocaine infusion, (h) 0 minutes post-lidocaine, (i) 30 minutes post lidocaine, (j) 1 hour post lidocaine. The stimulus consisted of a rotating full-field grating with a spatial frequency of 0.20 cycles/degree and a rotation speed of 1 cycle/60 seconds. Hue in polar maps represents the stimulus orientation (-90° to 90°, see inset) resulting in greatest activation at a given pixel. Brightness in polar maps indicates how strongly activated the pixel was by a stimulus at its preferred orientation. Black star indicates cannula placement. Arrows indicate area of significant map deterioration and recovery. Scale bar represents 2.5mm.
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Supplementary Figure 3. Computerized scoring of ocular dominance (OD) in cats with normal binocular vision. Data represent 1112 micro-electrode recordings in V1 from 6 critical period cats with normal binocular vision (11 hemispheres). The distribution is comparable to OD distributions previously generated with subjective ranking of neuronal responses.
