The Journal of Neuroscience, October 12, 2005, ():
Neuregulin-1 Reverses Long-Term Potentiation at CA1 Hippocampal Synapses
J. Neurosci. Kwon et al.
25: 9378
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplementary Figure 1. NRG-1 effects on LTP are blocked by the ErbB receptor inhibitor PD168393, which is structurally unrelated to PD158780. Field EPSPs (fEPSP) were recorded as described in Fig. 1 and the graph was normalized to mean baseline fEPSPs measured prior to the TBS. PD168393 (10 uM) was perfused 20 min following TBS (arrow) for 10 min prior to NRG-1?1 and for another 10 min in presence of 1 nM NRG-1?1 (n=5). Gray and black bar indicate duration of PD168393 and NRG-1?1 treatment, respectively. All traces are mean ± SEM.
- supplemental material
-
Supplementary Figure 2. NRG-1?1 has no effect on NMDAR-mediated currents at potentiated synapses. Ten minutes after LTP induction, AMPAR- and NMDAR-mediated EPSCs were measured at -70 and +40 mV, respectively, followed by incubation with CNQX, and CNQX plus NRG-1. (A) Top, Diagram of the time course and holding potentials used in the recordings. Bottom, Twenty averaged traces of EPSCs recorded at -70 and +40 mV in (1) ACSF, (2) ACSF+10 uM CNQX, and (3) ACSF+10 uM CNQX+1 nM NRG-1?. (B) Quantification of AMPAR (white) and NMDAR (black) normalized EPSC slopes. Traces are mean + SEM, n = 6.
- supplemental material
-
Supplementary Figure 3. Effect of NRG-1 on the surface expression of native AMPA and NMDA receptors in dissociated hippocampal neurons. Cultures were maintained in APV (APV), switched to 200 ?M glycine-containing media to induce chemLTP (Gly), and treated for 20 min with either NRG-1? (NRG) or NRG1?+PD158780 (NRG+PD) during chemLTP induction. Surface AMPARs and NMDARs were detected using antibodies against the extracellular domains of GluR1 and NR1, respectively.
