The Journal of Neuroscience, October 12, 2005, ():
Both Laminin and Schwann Cell Dystroglycan Are Necessary for Proper Clustering of Sodium Channels at Nodes of Ranvier
J. Neurosci. Occhi et al.
25: 9418
Supplemental data
Files in this Data Supplement:
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FigS1. The molecular organization of paranodes and juxtaparanodes in dystrophic mice is preserved.
Immunostaining of teased fibers from dystrophic (A-C, G-I) and wild type (D-F, J-L) sciatic nerves of adult mice. A-F, Dystrophic teased fibers double stained with an antibody against neurofascin 155 and 186 (A, green) and against myelin associated glycoprotein (MAG; B red) show appropriate colocalization of neurofascin and MAG at the paranode ( C, merge ) and staining of neurofascin at the node, similarly to wild type ( D, E and F respectively). G-L, Double staining of dystrophic and wild type teased fibers with antibodies against the paranodal marker Caspr (H and K, red ) and the Kv1.1 isoform of potassium channels (G and J, green ) confirms that the clustering of potassium channels is correctly concentrated at the juxtaparanode, and does not overlap with paranodes, both in dystrophic (I, merge) and in wild type (L, merge) fibers. Bar= 16 ?m.
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Fig. S2 The immature Nav 1.2 isoform is not abnormally expressed in dystrophic dy2Jdy2J or P0Cre ?1 sciatic nerves.
Immmunostaining of longitudinal sections of sciatic nerves from adult dystrophic mice (A, B) and wild type littermates (C, D); laminin P0Cre ?1 null mice (E, F), and wild type littermates (G, H); or optic nerve from a P11 wt mouse (I, L).
The fibers are double stained with anti-Nav1.2 antibody (black and white in A, C, E, G, I, red in the merge panels B, D, F, H, L) and an anti-Caspr antibody (green in the merge panels, B, D, F, H, L). The Nav1.2 isoform is found in optic nerves from control mice at P11 (arrows, I, L), but only occasionally in sciatic nerves from adult dystrophic (A, B), laminin ?1 P0Cre (E, F) and control mice (C-D, G-H). When found in adult nerves, the Nav1.2 isoform is usually associated with heminodes (inset in B, and asterisks in D, F).
