The Journal of Neuroscience, October 12, 2005, ():
Endocannabinoid Signaling Dynamics Probed with Optical Tools
J. Neurosci. Heinbockel et al.
25: 9449
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. UV-visibleabsorption spectrum of compound 4b before (solid line) and after (dashed line) 60-second exposure to 75 mW of the multi-line UV emission of an argon ion laser (BeamLok 2060-7S, Spectra-Physics Lasers, Mountain View, CA). The sample was prepared in 0.1 M Na phosphate buffer (pH 7.4).
- supplemental material
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Supplemental Figure 2. Photolysis kinetics of compound 4b measured by transient absorbance spectral change following laser flash photolysis. The absorbance at 430 nm of a solution of 4b in 0.1 M sodium phosphate (pH 7.4) was monitored. At time zero, a pulse of 355-nm light (8.6 ns FWHM, ~30 mJ) was delivered to the sample. In the upper panel, gray points are experimental data (average of 16 replicate experiments), and the solid black curve is the nonlinear least-squares single-exponential fit to the data. The two vertical dashed lines in the upper panel delimit the 45-ns window during which light scattering from the laser pulse and electronic instabilities of the photometric system contribute artifacts to the data; data within this window were not included in the least-squares analysis. The exponential time constant of the fitted decay curve is t = 106 ± 1 ns. The residuals of the fit are shown in the lower panel.
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Supplemental Figure 3. Example of an AMPA-mediated response mediated by photoreleased glutamate in a CA1 pyramidal cell in the slice. The Ncm-Glu concentration and laser flash parameters were the same as used in other figures, hence the onset of the AMPA response (~ 4 ms) illustrates that glutamate release and binding to receptors will not be rate-limiting for the mGluR-induced eCB responses shown in Figs. 7 and 8.
