The Journal of Neuroscience, October 12, 2005, ():
Interaction of SPIN90 with Dynamin I and Its Participation in Synaptic Vesicle Endocytosis
J. Neurosci. Kim et al.
25: 9515
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplemental Fig. 1
Preferential expression of synaptopHluorin in axonal compartment and spH fluorescence signal during firing of action potentials. (A-F) Hippocampal neurons that have been transfected with synaptopHluorin were fixed, permeabilized and immunostained with anti-MAP2, a dendritic marker, or anti-tau-1, an axonal marker, antibody. (G) Representative images of spH fluorescence changes in synaptic boutons. After 40 s, neurons were stimulated with 300 action potentials at 20 Hz. (H) Decay curve of spH fluorescence after cessation of stimulation. The dark line is a single exponential fit to the average fluorescence decay. Scale bars: 20 mm (A-F), 3 mm (G).
- supplemental material
-
Supplemental Fig. 2
(A) Hippocampal neurons were transfected with GFP-tagged SPIN90-specific siRNA, after which the lysates were immunblotted with anti-SPIN90 antibody. (B) Relative band intensity was measured and displayed as histograms.
