The Journal of Neuroscience, November 2, 2005, ():
Neuronal Differentiation in the Adult Hippocampus Recapitulates Embryonic Development
J. Neurosci. Espósito et al.
25: 10074
Supplemantal data
Files in this Data Supplement:
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SUPPLEMENTAL FIGURE S1. Development of passive and active membrane properties. A-C. Passive properties. Left panels: input resistance (A), membrane capacitance (B) and resting potential (C) of GFP+ neurons (green squares; N = 198) and GFP- neurons (blue triangles; N = 187) versus age. Right panels: cumulative distributions of the data shown on the left, where time intervals were color-coded (top) and data from GFP- neurons from all intervals were pooled (blue). D-F. Excitability. Left panels: peak spike amplitude (D), peak inward Na+ current (E) and steady-state outward K+current (F) versus age. Right panels: cumulative distributions (similar to A-C). Statistical comparisons were carried out for left panels; data of GFP+ neurons at each interval were compared against data of GFP- neurons averaged for the whole time course, using Kruskall-Wallis followed by a post-hoc Dunn´s test for multiple comparisons. Data are mean ± SEM. (*),(x) and (#) stand for p<0.05, p<0.01 and p<0.001.
- supplemental material
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SUPPLEMENTAL FIGURE S2. Kinetic properties of GABAergic spontaneous PSCs. Scatter plots of decay versus rise time from spontaneous PSCs recorded at -80 mV in the presence of kyn (4 mM) from: (A) GFP+ neurons bearing GABAergic afferents only (data pooled from 6 neurons) and (B) more mature neurons with both GABAergic and glutamatergic inputs (N = 9). C. Recordings obtained from GFP- DGCs were used as controls for comparison (N = 11). D. Recordings of spontaneous PSCs from GFP- neurons under symmetrical Cl- at Vhold = 0 mV (N = 5). Relative frequencies of spontaneous PSCs versus rise time are shown in blue.
