The Journal of Neuroscience, November 2, 2005, ():
A Small-Molecule-Inducible Nrf2-Mediated Antioxidant Response Provides Effective Prophylaxis against Cerebral Ischemia In Vivo
J. Neurosci. Shih et al.
25: 10321
Supplemantal data
Files in this Data Supplement:
- supplemental material -
Supplemental table.
- supplemental material
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Supplementary Figure 1. TBHQ stability in mini-osmotic pumps. The 1 mM tBHQ solution (in PBS) was loaded into an Alzet mini-osmotic pump and maintained at 37oC within a PBS filled eppendorf tube for 4 days. The remaining solution was extracted from the pump after this time and used in a bioassay of neuronal viability during oxidative glutamate toxicity as described previously (Shih et al., 2003). The tBHQ solution was diluted to a final concentration of 20 mM in culture media. The tBHQ solution remained active within the pump for at least 4 days, since the extracted solution protected neurons as well effectively as freshly prepared tBHQ. Data represent the mean + SEM collected from 3 independent experiments.
- supplemental material
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Supplementary Figure 2. Effect of dietary tBHQ administration on permanent focal ischemia. Nrf2 colony mice were fed 1% tBHQ or control diet for 1 week and subjected to permanent focal ischemia. TBHQ feeding was continued for 1 additional week after stroke and the mice were then sacrificed for evaluation of infarct size. No significant difference was observed between control and tBHQ diet with any genotype. Data represent the mean + SEM from at least 4 mice for each genotype and treatment group. Body weight and core temperatures are reported in the Supplementary table.
