The Journal of Neuroscience, November 9, 2005, ():
Migration from a Mitogenic Niche Promotes Cell-Cycle Exit
J. Neurosci. Choi et al.
25: 10437
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Dissociated granule cell precursors are introduced into an organotypic slice.
Schematic illustrates the slice overlay assay. Granule cell precursors are purified from P6 GFP transgenic mice (green circles). They are introduced on a 250µm-thick organotypic slice. Introduced precursors distribute randomly in all layers of the cerebellum. EGL, external granule cell layer (black circles); ML, molecular layer; PcL, Purkinje cell layer (black ovals); IGL, internal granule cell layer; WM, white matter.
- supplemental material
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Supplemental Figure 2. Introduced precursors proliferate preferentially in the EGL and the WM.
Proliferative indices in all layers. The proliferative index is calculated by dividing the number of GFP-BrdU double-positive cells in a layer by the number of GFP+ cells in the layer. Introduced precursors show increased proliferative index in the WM as well as in the EGL. The EGL has a 3-fold higher proliferative index than the IGL, and the WM has a 3.45-fold higher proliferative index than the IGL. *p<0.05 (n=8, 4 experiments, Student’s t-test)
- supplemental material
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Supplement 3: Table. Blocking CXCR4 with AMD 3100 does not have an effect on proliferation of introduced precursors.
Slices with introduced precursors are cultured in the presence of AMD 3100, a small molecule inhibitor of CXCR4 (a range of concentrations used, data shown at 1.5ng/ml). AMD 3100 does not have an effect on proliferation of introduced precursors.
