The Journal of Neuroscience, November 16, 2005, ():
Lamina Propria and Olfactory Bulb Ensheathing Cells Exhibit Differential Integration and Migration and Promote Differential Axon Sprouting in the Lesioned Spinal Cord
J. Neurosci. Richter et al.
25: 10700
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Transplantation approach for direct or rostro-caudal introduction of OECs into dorsolateral funiculus lesion. Following a crush of the dorsolateral funiculus between C3 and C4, OECs are injected (1) directly into the crush at depths of 0.7, 1.0 and 1.5 mm or (2) rostrocaudally at depths of 0.6 and 1.2mm measured from the surface of the spinal cord. Rostral and caudal injection points were 1mm away from the lesion site. 75-90,000 cells were transplanted into each rat, and cells were evenly distributed between sites.
- supplemental material
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Supplemental Figure 2. Substance P, Calcitonin Gene Related Peptidergic, Serotonergic, and tyrosine hydroxylase positive axons retract from the lesion site twenty-four hours following injury.
Visualizing the lesion site (anti-GFAP; blue) allows us to test the proximity of axon subtypes (red) 24 hours following crush of the dorsolateral funiculus. 24 hours after lesion, subP positive axons in (A) control and (E) OEC treated rats are retracted100μm (arrowhead) from the site of injury (asterisk). CGRP-positive axons are not present at this level in (B) control or (F) OEC treated rats 24 hours after injury, where they are found at 28 days. Serotonergic (5HT) axons in (C) control and (G) rostrocaudal OEC treated rats are not visible crossing the crush site (asterisk) and many are retracted (minimum retraction denoted by arrowhead). (D,H) Tyrosine hydroxylase (TH)-positive axons are retracted (arrowheads) approximately 250μm from the crush site (asterisk) 24 hours after injury in control and OEC treated rats. LP Direct n=3, LP R/C n=4, OB R/C n=4, Control R/C n=4. Scale bars= 250μm.
