The Journal of Neuroscience, December 7, 2005, ():
Noradrenaline Triggers Multivesicular Release at Glutamatergic Synapses in the Hypothalamus
J. Neurosci. Gordon and Bains
25: 11385
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1 NA-induced bursts of mEPSCs display multimodal amplitude histograms. (A) Voltage clamp trace depicting a short burst of mEPSCs after a 2 s focal application of NA into the dendritic region of the magnocellular neurons. (B) Frequency plot of mEPSCs from the same cell in A using the same time scale. The frequency of mEPSCs in the burst is many fold higher than the background frequency. (C) Control amplitude distribution from the same cell above; 70 events. (D) Amplitude distribution generated from all of the mEPSCs in the NA-induced burst; 70 events. From these two distributions one can see a single mode in the control data and the addition of multiple, equidistant modes in the NA-induced burst. Scale bars: 50pA, 5s
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Supplementary Figure 2 10kHz sampling and 1kHz Bessel filtering do not produce a positive rise time versus amplitude relationship to artificial currents. (A) Seven incremental 2mV voltage steps were applied to a model cell in voltage clamp (bottom). The generated step artifacts ranged in amplitude from 20 to 150pA (top). (B) The 10 to 90 rise time of each artifact was calculated and plotted against its amplitude. There was no significant difference between the rise times of any artifact, suggesting that our sampling and filtering settings do not produce an artificial relationship between event rise time and amplitude. Scale bars top: 50mV, 25ms; Scale bars bottom: 8mV, 25ms
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Supplementary Figure 3 When larger mEPSCs are the result of postsynaptic AMPA current enhancement, γDGG does not display preferential attenuation of small versus large events. (A) Post NA mEPSCs taken from the 50th percentile and the 95th percentile of the raw amplitudes (left). Post NA mEPSCs taken from the 50th percentile and the 95th percentile of the raw amplitudes in the presence of γDGG (500μM) (middle). Scaling the γDGG events demonstrates that (DGG shows no preferential effects on either percentile group (right). (B) Summary bar graph from manuscript figure 9 showing the fractional amplitude of mEPSCs remaining in the presence of (DGG during NA (0.81 ± 0.03, n=6), control (0.65 ± 0.03, n=5, p<0.01) and post NA (0.62 ± 0.02, p<0.01, n=5). Scale bars: 10pA, 5ms
