The Journal of Neuroscience, December 14, 2005, ():
Distinct Subunits in Heteromeric Kainate Receptors Mediate Ionotropic and Metabotropic Function at Hippocampal Mossy Fiber Synapses
J. Neurosci. Ruiz et al.
25: 11710
Supplemental data
Files in this Data Supplement:
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Supplemental Figure 1: Modulation of IsAHP by KAR activation and the involvement of a metabotropic pathway.
(A) Input resistance (mean ± s.e.m.) plotted against time (n = 4), showing no change evoked by bath perfusion of 50 nM KA. (B) Sample traces show the average of 5 consecutive IsAHP recordings in neurons prior and after application of 50 nM KA in the presence of NBQX (50 µM). Summary bar chart showing that prior application of 20 µM CNQX (n = 3) or 50 µM NBQX (n = 5) blocked the effect of 50 nM KA on IsAHP. (C) KA-induced inhibition of IsAHP requires PTX-sensitive G-protein coupling and PKC activation. Bar chart illustrating the attenuated KA-induced inhibition of IsAHP in slices treated with calphostin C (1 µM, 2 – 6 hours; inhibition of 26 ± 7 %, n = 5) or perfused with NEM (50 µM, 20 minutes; inhibition of 28 ± 12 %, n = 9). Sample traces show the average of 5 consecutive traces prior and after application of 50 nM KA in neurons recorded from calphostin C or NEM-treated slices. (D) Bar charts of IsAHP characteristics obtained from wild-type, GluR6-/- (n = 9) and KA2-/- (n = 10) mice showing no significant differences in peak amplitude (pA), decay time constant (s) and charge (nC). Recordings were obtained with TTX (1 µM), TEA (5 mM), PTX (100 µM), APV (50 µM), CGP 55845 (5 µM), LY 341495 (100 µM), and GYKI 53655 (50 µM). The bar charts show mean ± s.e.m values. * p < 0.05.
