The Journal of Neuroscience, December 14, 2005, ():
Hedonic Hot Spot in Nucleus Accumbens Shell: Where Do µ-Opioids Cause Increased Hedonic Impact of Sweetness?
J. Neurosci. Peciña and Berridge
25: 11777
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Figure S1. Horizontal & Coronal Maps of Hedonic Hotspot
A. Two-level maps of positive hedonic hotspot and coldspot in horizontal plane. ‘Dorsal section’ maps show rostral/dorsal localization in medial shell of hedonic enhancement effects in increasing number of positive ‘liking’ reactions to sucrose (each DAMGO dose mapped separately). Plume symbols are predominantly contained within the medial shell of nucleus accumbens, indicating that Fos plumes did not penetrate deeply into adjacent brain structures. A ‘ventral plane’ shows weaker hedonic ‘hotspot’ and ‘coldspot’ localization effects. B. Coronal maps of DAMGO effects on positive hedonic reactions to sucrose, plotted separately for 0.01 µg and 0.1 µg doses. Symbols as in Figure 2.
- supplemental material
-
Figure S2. Hedonic vs Intake Contrast: Dose-Response Maps
Breakdown of hedonic/intake contrast map (Fig 4A) into separate 0.01 µg and 0.1 µg doses of DAMGO. Hedonic enhancement effects on positive hedonic ‘liking’ reactions to sucrose (shown in red/orange/yellow) versus stimulation of food intake (green/blue). A mapping threshold was set at >200% increase over vehicle control levels for both hedonic and intake effects, requiring DAMGO to at least double hedonic reactions or food intake for a site to be colored. Hedonic effects are mapped on top of intake effects. Red/yellow hedonic hotspot shows where DAMGO caused both increased hedonic ‘liking’ reactions and increased food intake. Blue/green shows areas where DAMGO increased food intake but failed to increase hedonic ‘liking’ reactions to taste.
- supplemental material
-
Figure S3. Hedonic vs Aversive Contrast: Dose-Response Maps
Breakdown of affective hotspot/coldspot contrast into separate 0.01 µg and 0.1 µg DAMGO doses. Hedonic hotspot (red/yellow) shows enhancement of positive hedonic reactions to sucrose taste, whereas affective cold spot shows where DAMGO suppressed negative aversive reactions to quinine taste (purple). Mapping threshold for hedonic hotspot required DAMGO at a site to double the number of positive reactions elicited by sucrose taste. Threshold for mapping coldspot suppression required DAMGO at that site to cut in half the number of aversive reactions elicited by quinine taste (both combined for each rat). Hedonic enhancement was mapped on top of aversive suppression (i.e., aversive suppression extends under red/yellow zone).
- supplemental material
-
Movie 1: Affective taste reactivity video.
Video examples of affective 'liking' and 'disliking' reactions to tastes. Rat is viewed from underneath transparent floor as tastant solutions are painlessly infused into its mouth through a previously-implanted oral cannula. Sucrose taste elicits positive hedonic or 'liking' reactions from n rats (e.g., rhythmic tongue protrusions). Quinine taste elicits negative aversive or 'disliking' reactions (e.g. gapes).
