The Journal of Neuroscience, February 9, 2005, ():
Functional Thalamocortical Synapse Reorganization from Subplate to Layer IV during Postnatal Development in the Reeler-Like Mutant Rat (Shaking Rat Kawasaki)
J. Neurosci. Higashi et al.
25: 1395
Supplemental data
Files in this Data Supplement:
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Calretinin (green) and chondorotin sulfate proteoglycan (red) immunoreactivities revealed the inverted position of the subplate cells in SRK. Panels A, C, E and G are from WT and B, D and F and H are from SRK. A, B E and F are at stage E19 and C, D, G and H at P0. In WT, calretinin revealed numerous immunoreactive cells in subplate and marginal zone, but also occasionally some in cortical plate (A) at E19. In the SRK most of the CR immunoreactive cells were found in the superplate at this stage. By P0 most of the CR immunoreactive cells were still found in subplate in WT, but they appeared in the cortex in larger numbers (C) compared to E19. In SRK (D) the heaviest staining was in the superplate at P0. Arrows depict CR positive cells in subplate (C) and superplate (D). At E19 chondorotin sulfate proteoglycan immunoreactivity was the strongest in subplate (E) and in deep cortical layers in WT at P0 (G). In contrast, the antibody stained superficial cortical layers in SRK at E19 (F) and P0 (H). The CR and chondroitin sulfate proteoglican staining patterns further support the notion that subplate cells are in the superplate in the SRK. Nevertheless, none of these stains labeled subplate cells selectively and exclusively. Scale bars: 50 ƒÊm for all panels.
