The Journal of Neuroscience, February 16, 2005, ():
Overexpression of GD1a Ganglioside Sensitizes Motor Nerve Terminals to Anti-GD1a Antibody-Mediated Injury in a Model of Acute Motor Axonal Neuropathy
J. Neurosci. Goodfellow et al.
25: 1620
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1A: Topical application of anti-GD1a-KLH mAbs
Immunohistological analysis of anti-GD1a-KLH mAb and complement fixation.
A Topical application of anti-GD1a-KLH mAbs to diaphragm. Median, interquartile range and 1.5 times the interquartile range are shown as in Figure 3 (see Materials and Methods). Levels of IgG (white) or C3c (hatched) deposition at NMJs in sections of WT, GD3s-/- and GalNAcT -/- diaphragm are shown. Highest levels of IgG and C3c were seen in the GD3s-/- mouse. *** = p<0.001 compared with WT.
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Supplementary Figure 1B: Effects of anti-GD1a-KLH mAbs. Immunohistological analysis of anti-GD1a-KLH mAb binding, complement fixation and destruction of neurofilament (NF).
B Ex vivo application of anti-GD1a-KLH mAbs to GD3s-/- hemi-diaphragm preparations. MOG-35 was used as a positive control to enable comparison with anti-GD1a-LOS mAbs. Ringer’s solution, MOG-35 or anti-GD1a-KLH mAb was applied followed by a source of complement. Subsequent IgG (white), C3c (hatched) and NF (black) levels were calculated for each genotype. Anti-GD1a-KLH mAbs bound to the NMJ, fixed complement and caused a loss of NF in the GD3s-/- mouse. None of these mAbs caused lysis of pSCs. *** = p<0.001 compared with Ringer’s control. Median values for individual mAbs are shown in numbered circles.
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Supplementary Figure 1C: Effects of anti-GD1a positive mouse sera.
Immunohistological analysis of mouse serum binding, complement fixation and destruction of neurofilament (NF).
C Ex vivo application of anti-GD1a-KLH-positive serum to GD3s-/- hemi-diaphragm preparations. Heat-inactivated non-immunised control sera, MOG-35 or heat-inactivated anti-GD1a-KLH-positive sera was applied followed by a source of complement. IgG (white), C3c (hatched) and NF (black) levels were calculated for each genotype. Anti-GD1a-KLH-positive sera bound to the NMJ, fixed complement and caused a NF loss in the GD3s-/- mouse. None of these sera caused lysis of pSCs. Median values for sera isolated from separately immunised mice are shown as 1, 2 and 3. *** = p<0.001 compared with control sera.
