The Journal of Neuroscience, February 16, 2005, ():
A Novel Pathway for Presynaptic Mitogen-Activated Kinase Activation via AMPA Receptors
J. Neurosci. Schenk et al.
25: 1654
Supplemental data
Files in this Data Supplement:
- supplemental material
-
6DIV hippocampal neurons were monitored by means of calcium imaging. A: Stimulation with 50|*mu*|M AMPA in calcium free KRH did not lead to significant calcium increase either at the growth cone nor somatodendritically (black trace). All the experiments described in this study have been carried out under this experimental conditions. In the presence of 100|*mu*|M cyclothiazide, stimulation with AMPA led to a gradual increase of the cytosolic calcium (B). A prominent calcium influx was detectable following addition of external calcium.
- supplemental material
-
The vesicular neurotransmitter transporters vGlut2 and vGAT but not vGlut1, are present on vesicles immunoisolated from growth cone particles using an antibody directed against synaptotagmin (A), thus suggesting that recycling of immature synaptic vesicles in the growth cone is already associated with neurotransmitter release. AMPA receptor subunits GluR2,3 are much more abundant on vesicles immunoisolated from growth cone particles compared to vesicles immunoisolated from mature synaptosomes (B).
.gif?ad=17923&adview=true)
