The Journal of Neuroscience, March 2, 2005, ():
Shal and Shaker Differential Contribution to the K+ Currents in the Drosophila Mushroom Body Neurons
J. Neurosci. Gasque et al.
25: 2348
HTML Page - index.htslp
Supplemental Figures
Files in this Data Supplement:
- Supplemental Fig. 1
-
Supplemental Figure S1. GFP-expression in the MBNs requires the 7A2 driver. A, The 7A2 construct was generated by cloning a 10-kb fragment that includes the transcription initiation site of the fruitless (fru) gene into the pPTGAL vector. This fragment is flanked by SalI sites and was cloned into the BamHI site of pPTGAL rendered compatible after a two base-pair fill-in reaction. The insertion of the transposon was mapped to the second chromosome. B, C. Third intar larval brains were dissected in PBS, fixed in 4 % paraformaldehide and slightly flattened by a glass coverslip. Bright field images of whole-mounts are shown to the left, whereas maximal intensity confocal projections of the same preparations are shown to the right. The brains are oriented with the anterior face upwards and dorsal at the top. B, 7A2-Gal4/UAS-GFP line. C, UAS-GFP/UAS-GFP parental line. GFP expression depends on the Gal4 enhancer trap in the 7A2 line. Whether the enriched expression of Gal4 in the Mushroom Body neurons is actually driven by the fru fragment that the 7A2 construct carries or by an endogenous enhancer trapped by this construct remains unknown. The Gal4 expression pattern is not related to the fru expression pattern since GFP expression and Fru immunolocalization do not correlate (data not shown).
- Supplementary Fig. 2
-
Supplemental Figure S2. GFP MB-enriched expression appears as early as the first instar larval stage. A, The same 7A2-Gal4/UAS-GFP larval brain examined in Supplemental Figure S1 was overexposed to reveal weak GFP-expression. Under this conditions several GFP-expressing cells are revealed in the ventral ganglion. The identity of these cells was not investigated. Ventral ganglia were removed from the brains used in the RT-PCR and electrophysiological experiments, thus reducing contamination of non-MB fluorescent cells. B, Amplification of the cerebral lobes of the brain displayed in A. Arrows point to GFP-expressing neuronal somata outside the MB region. In this preparation we could count 12 non-MB GFP-expressing cells (arrowheads point to cluster of three neurons), plus the large cells in the par intercebralis (asterisk). C, High-resolution image of the MB neuronal somata and calyx. This image reveals that the GFP-signal detected in the MB-region comes from a widespread expression of GFP in the MBNs rather than from a high GFP-expression restricted to a small subset of MBNs. D, First instar larval brain (10-14 hrs after hatching) showing expression of GFP in the MBNs. Neurons in the ventral ganglion were also stained (not shown).
