The Journal of Neuroscience, January 4, 2006, ():
Capture of the Late Phase of Long-Term Potentiation within and across the Apical and Basilar Dendritic Compartments of CA1 Pyramidal Neurons: Synaptic Tagging Is Compartment Restricted
J. Neurosci. Alarcon et al.
26: 256
Supplemental data
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Supplemental tables
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Supplementary Figure 1. Interactions between two pathways in the basilar compartment. In ten experiments where the configuration of the electrodes was similar to that used in figure 2 but with a paired-pulse facilitation across pathways larger than 120%, induction of L-LTP in S1 produced either a decrease (A: S2, 20-30 min mean amplitude=85±3.7%, n=6) or an increase (B: S2, 20-30 min mean amplitude=170±9%, n=4) of the baseline in S2. Under those circumstances we failed to observe capture of L-LTP. Perhaps, the detection of capture of L-LTP could have been masked or occluded by the depression or potentiation of the baseline, respectively. S1 and S2 represent two independent inputs to the basal dendritic layer of the CA1 area.
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Supplementary Figure 2. Failure for Transcompartmental capture of L-LTP. A. After induction of L-LTP by 4 trains of 100 Hz (4 HFS) in basilar dendrites (S1, closed squares), capture of L-LTP does not occur in the proximal apical dendrites using 1 train of 100 Hz stimulation (1 HFS), n=5. B. After induction of L-LTP in basilar dendrites capture of L-LTP does not occur in the distal apical dendrites, n=5. These data suggest that the distance to the nucleus is not a factor for the failure to observe transcompartmental capture. S1 and S2 represent two independent inputs to the basal and apical dendritic layers of the CA1 area, respectively.
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Supplementary Figure 3. Intracompartmental apical capture of L-LTP. A. Input-specific E-LTP induced by 1 train of 100 Hz (1 HFS) in a proximal apical pathway (S1) does not affect baseline stability of a second distal apical pathway (S2). B. Intracompartmental apical capture of L-LTP is not enhanced by the proximity of the capturing pathway to the nucleus (S1, open circles), n=6. C. Once L-LTP is evoked in the proximal dendrites (4 HFS, S2, closed squares), it is possible to capture L-LTP in the distal dendrites using 1 train of 100 Hz stimulation (1 HFS, closed circles), n=6. S1 and S2 represent two independent inputs to the apical dendritic layer of the CA1 area.
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Supplementary Figure 4. Given the crucial role of temperature on synaptic metabolism, we tested compartment-specific marking and transcompartmental capture of L-LTP at a temperature of 34 degrees Celsius and confirmed further our studies carried out at 28 degrees Celsius. A. Failure to observe transcompartmental capture of L-LTP with 1 train of 100 Hz (1 HFS) in apical dendrites (S2 pathway, closed circles), n=5. B. Capture of LTP across dendritic compartments can be elicited by 2 trains of 100 Hz (30 sec inter train interval, 2 HFS) in basal dendrites (S1, closed squares), n=5. S1 and S2 represent two independent inputs to the basal dendritic layer of the CA1 area.
