The Journal of Neuroscience, March 8, 2006, ():
Cocaine Self-Administration in Mice Is Inversely Related to Phosphorylation at Thr34 (Protein Kinase A Site) and Ser130 (Kinase CK1 Site) of DARPP-32
J. Neurosci. Zhang et al.
26: 2645
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure. Each of the phosphorylation sites of the phosphoprotein DARPP-32 is shown with its respective phosphorylating enzyme. DARPP-32 is phosphorylated at Thr34 by PKA, at Thr75 by Cdk5, at Ser97 by CK2, and at Ser130 by CK1. P-Thr34 is preferentially dephosphorylated by PP-2B; P-Thr75 is preferentially dephosphorylated by PP-2A; P-Ser130 is preferentially dephosphorylated by PP-2C; the phosphatase for P-Ser97 is not yet characterized. Phosphorylation of Ser97 of DARPP-32 by CK2 increases the rate of phosphorylation of Thr34 by PKA; phosphorylation of Ser130 of DARPP-32 by CK1 decreases the rate of dephosphorylation of P-Thr34 by PP-2B. Phosphorylation at Thr75 converts DARPP-32 into an inhibitor of PKA, reducing its ability to phosphorylate DARPP-32 and other substrates (blue arrows indicate positive effect; red arrows indicate negative effect). Phosphorylation of Thr34 converts DARPP-32 into a potent inhibitor of PP-1. This figure is modified from Svenningsson et al., 2004.
