The Journal of Neuroscience, March 8, 2006, ():
Role of Efficient Neurotransmitter Release in Barrel Map Development
J. Neurosci. Lu et al.
26: 2692
Supplemental data
Files in this Data Supplement:
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Supplemental materials
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Figure 1. Effects of kynurenic acid on the paired-pulse response of AMPA-EPSCs
(A, B) Example traces (average of ten sweeps) of AMPA-EPSCs triggered by paired stimuli in the presence of regular Ringer (blue traces) and then after the addition of 0.5 mM kynurenic acid (green traces). Kynurenic acid is a non-specific glutamate receptor antagonist, which at this concentration will partially block AMPARs. (B) Summary of P4-6 AMPA-PP ratios with various inter-stimulus intervals (ISIs). The ctrl PP ratios are: in normal Ringer: PP50, 0.92 ± 0.08, PP100, 0.81 ± 0.06, PP500, 0.81 ± 0.07 in kynurenic acid-Ringer: PP50, 1.03 ± 0.17, PP100, 0.77 ± 0.07, PP500, 0.50 ± 0.04, n=8. The variability of AMPA-PP ratios with short ISIs in the presence of kynurenic acid is much greater than the distribution of PP ratios in normal Ringer, while there is no increase in the variability for 500 msec ISIs.
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Figure 2. Effects of L-AP5 on the paired-pulse response of NMDA-EPSCs
(A) Application of L-AP5 (800 uM) in a control neuron causes a decrease in NMDA-EPSC amplitude and an increase in paired-pulse response. Example traces (average of ten sweeps) of NMDA-EPSCs triggered by paired stimuli in the presence of regular Ringer (blue traces) and after the addition of 800 uM L-AP5 (green traces). Lower example traces show the same response scaled to the response to the first stimulus, demonstrating that paired-pulse increases in the presence of L-AP5. (B) The same experiment conducted in barrelless mice shows a similar effect, with a decrease in the amplitude of NMDA-EPSCs in the presence of L-AP5 (800 uM) and an increase in the paired-pulse response. On average, the paired-pulse ratio is still significantly larger in brl mice compared to control in the presence of L-AP5 (see Figure 1F).
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Figure 3. Normal NMDAR kinetics in barrelless thalamocortical connections.
(A) Example traces of NMDA currents (average of 10 sweeps) from brl and ctrl mice. Bottom panel shows the two current averages rescaled to the same amplitude, revealing similar NMDAR kinetics between ctrl and brl neurons. (B) Weighted time constant (calculated as described in Lu et al., 2001) for the NMDAR current at P3-6 and P9-11 age group (P3-6, ctrl, 261±20 msec, n=14; brl, 233±15 msec, n=6; P9-11, ctrl, 160±15 msec, n=7; brl, 171±28 msec, n=7).
