The Journal of Neuroscience, March 15, 2006, ():
Remodeling of Synaptic Structure in Sensory Cortical Areas In Vivo
J. Neurosci. Majewska et al.
26: 3021
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplementary Figure 1. Confirming the location of imaged neurons.
A. Examining the location of imaged neurons in histological sections. Following imaging, red tracer was injected into the imaged area based on the blood vessel pattern. The animal was perfused and the imaged area identified in fixed section. Injections are shown in red and the extent of the sensory area is demarcated with arrows. Left: primary visual cortex (V1b, binocular portion of V1). Middle: primary auditory cortex (A1). Right: primary somatosensory cortex (S1bf, barrel field of S1). B. In some cases the tracer was allowed to transport for 2 days. To confirm imaging in primary auditory cortex (arrows) retrogradely labeled neurons were observed in the MGN (delineated in broken red oval). C. Synapsin staining colocalizes with putative presynaptic sites. Bottom panel shows the overlay of GFP-stained axons (top panel) and synapsin staining (middle panel) imaged in fixed sections. Arrowheads indicate putative presynaptic terminals morphologically similar to those imaged in vivo.
- supplemental material
-
Supplementary Figure 2. Spine class composition in different cortical areas.
A. Developmental profile of spine class composition in the mouse visual cortex. At the ages studied (P28-P61) most spines had adult mushroom morphologies. As the animals enter adulthood (between P28 and P40), the percentage of thin spines decreases significantly with a concomitant increase in stubby spines (p<0.05). Additionally filopodia are rare at these ages but become increasingly infrequent after P28. B. The same developmental profile is maintained in all sensory cortical areas studied suggesting that maturation of cortical spine morphology is not regulated in a cortical area-specific manner.
