The Journal of Neuroscience, March 15, 2006, ():
Double Patch Clamp Reveals That Transient Fusion (Kiss-and-Run) Is a Major Mechanism of Secretion in Calf Adrenal Chromaffin Cells: High Calcium Shifts the Mechanism from Kiss-and-Run to Complete Fusion
J. Neurosci. Elhamdani et al.
26: 3030
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental figure 1. Model of transient and full fusion of DCVs evoked by physiological stimulation in calf AC cells. (Mode 1) at low stimulation (low Cai), DCVs connect transiently with the plasma membrane through a fusion pore with a low conductance and longer opening time. Retrieval of these vesicles is a reversal of the fusion reaction. In this mode of release the vesicle may undergo few rounds of release with no need to undock.
(Mode 2) a moderate stimulation (medium Cai) speeds up the closure and increases the conductance of the fusion pore. This type of secretion is very efficient during the brief opening time of the fusion pore. As kiss-and-run events and RE are both abrogated by high Cai and Sr2+ as charge carrier, the brief opening time of the fusion pore is most likely linked to the Ca2+ and dynamin-1-dependent RE. As these vesicles are retrieved intact, they will get refilled by transmitter from the cytoplasm and get docked and primed again before undergoing another round of secretion.
(Mode 3) At sustained stimulation (high Cai), the fusion pore will open briefly at higher conductance. The absence of dynamin-1 rings around the neck of the vesicle will cause the fusion pore to expand until full collapse of the vesicle with plasma membrane. In this condition, vesicles are retrieved by SE through the clathrin and dynamin-2-dependant endocytosis and recycle through the endosomal compartment.
