The Journal of Neuroscience, March 15, 2006, ():
Spatial Heterogeneity in Oligodendrocyte Lineage Maturation and Not Cerebral Blood Flow Predicts Fetal Ovine Periventricular White Matter Injury
J. Neurosci. Riddle et al.
26: 3045
Supplemental data
Files in this Data Supplement:
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Supplemental material
- supplemental material
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Supplemental Figure 1. Focal PVWM lesions contain activated microglia and phagocytic macrophages identified with isolectin B4 or Ham 56. Panels A-C show higher power details of immunofluorescent staining for isolectin B4 in the control PVWM (A) relative to PVWM lesions (arrowheads) generated after 37- (B) or 45-min (C) of ischemia. Microglia/macrophage-rich lesions were also confirmed using a Ham 56 antibody (D-F). Asterisk denotes apex of lateral ventricle. Scale Bars: A-F, 200 µm.
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Supplemental Figure 2. Time course for appearance of TUNEL-labeled nuclei in frontal PVWM (A) and cerebral cortex (B) after 30 or 45 min of global ischemia (n=4 animals per group at 24 h or 72 h survival). The density of TUNEL-labeled nuclei was consistently higher 24 h after global ischemia than after 72 h. In A, B, # p<0.001, 30 min H/I 24 vs. 72 h recovery; ✝ p<0.05, 45 min H/I 24 vs. 72 h recovery: unpaired two-tailed t tests.
