The Journal of Neuroscience, March 22, 2006, ():
Chemokines Regulate the Migration of Neural Progenitors to Sites of Neuroinflammation
J. Neurosci. Belmadani et al.
26: 3182
Supplemental data
Files in this Data Supplement:
- supplemental material -
Table 1 (Supplemental data): Multiplex immunoassay determinations of media cytokine and chemokine levels (pg/mg protein) after 16 hours of treatment with gp120, LPS, or β-amyloid.
- supplemental material
-
Figure S1 (supplemental material): Protocol illustrating the use of hippocampal slice cultures to assess the ability of neural progenitors to migrate in response to a neuroinflammatory focus. A) Bright field illustration of hippocampal slice culture showing its subregions and cellular organization after 7 days in culture. C and C' illustrate EGFP and PKH26 labeling of neural progenitors (neurospheres). B): Green dots illustrate the site of transplantation of EGFP expressing cells, red dots the site of injection of the inflammatory stimulus. Abbreviations: ca1, ca3 subfields; fi, fimbria; dg, dentate gyrus; S, subiculum. Scale bar = 500μm.
